Identification of cell surface glycoproteins by periodate-alkaline phosphatase hydrazide

Zvi Keren; Gideon Berke; Jonathan M. Gershoni

doi:10.1016/0003-2697(86)90244-7

Identification of cell surface glycoproteins by periodate-alkaline phosphatase hydrazide

Zvi Keren, Gideon Berke, Jonathan M. Gershoni^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

A novel method for the detection of cell surface glycoconjugates has been developed. Cells are subjected to mild surface oxidation of vicinal hydroxyls with sodium periodate. Afterward, cellular proteins are resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and then probed with alkaline phosphatase hydrazide. The technique is sensitive, reproducible, and inexpensive. It obviates the need for radiolabeled NaBH₄ and the subsequent processing of polyacrylamide gels for fluorography. Results are easily obtained in a matter of a few hours.

Original language	English
Pages (from-to)	182-187
Number of pages	6
Journal	Analytical Biochemistry
Volume	155
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(86)90244-7
State	Published - 15 May 1986
Externally published	Yes

Keywords

Alkaline phosphatase hydrazide
cell surface labeling
glycoproteins
periodate oxidation
protein blot

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10.1016/0003-2697(86)90244-7

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title = "Identification of cell surface glycoproteins by periodate-alkaline phosphatase hydrazide",

abstract = "A novel method for the detection of cell surface glycoconjugates has been developed. Cells are subjected to mild surface oxidation of vicinal hydroxyls with sodium periodate. Afterward, cellular proteins are resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and then probed with alkaline phosphatase hydrazide. The technique is sensitive, reproducible, and inexpensive. It obviates the need for radiolabeled NaBH4 and the subsequent processing of polyacrylamide gels for fluorography. Results are easily obtained in a matter of a few hours.",

keywords = "Alkaline phosphatase hydrazide, cell surface labeling, glycoproteins, periodate oxidation, protein blot",

author = "Zvi Keren and Gideon Berke and Gershoni, {Jonathan M.}",

note = "Funding Information: ACKNOWLEDGMENTS The study was supported by funds from Keren Yeda and the Bat-Sheva de Rothschild Foundation for the Advancement of Science in Israel. The authors thank Dr. Meir Wilchek for his support and critical discussion, Jean-Michel Lebleautte for comments, and Ms. Pamela Rubinstein for her assistance in preparing this manuscript.",

year = "1986",

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doi = "10.1016/0003-2697(86)90244-7",

language = "אנגלית",

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T1 - Identification of cell surface glycoproteins by periodate-alkaline phosphatase hydrazide

AU - Keren, Zvi

AU - Berke, Gideon

AU - Gershoni, Jonathan M.

N1 - Funding Information: ACKNOWLEDGMENTS The study was supported by funds from Keren Yeda and the Bat-Sheva de Rothschild Foundation for the Advancement of Science in Israel. The authors thank Dr. Meir Wilchek for his support and critical discussion, Jean-Michel Lebleautte for comments, and Ms. Pamela Rubinstein for her assistance in preparing this manuscript.

PY - 1986/5/15

Y1 - 1986/5/15

N2 - A novel method for the detection of cell surface glycoconjugates has been developed. Cells are subjected to mild surface oxidation of vicinal hydroxyls with sodium periodate. Afterward, cellular proteins are resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and then probed with alkaline phosphatase hydrazide. The technique is sensitive, reproducible, and inexpensive. It obviates the need for radiolabeled NaBH4 and the subsequent processing of polyacrylamide gels for fluorography. Results are easily obtained in a matter of a few hours.

AB - A novel method for the detection of cell surface glycoconjugates has been developed. Cells are subjected to mild surface oxidation of vicinal hydroxyls with sodium periodate. Afterward, cellular proteins are resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and then probed with alkaline phosphatase hydrazide. The technique is sensitive, reproducible, and inexpensive. It obviates the need for radiolabeled NaBH4 and the subsequent processing of polyacrylamide gels for fluorography. Results are easily obtained in a matter of a few hours.

KW - Alkaline phosphatase hydrazide

KW - cell surface labeling

KW - glycoproteins

KW - periodate oxidation

KW - protein blot

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DO - 10.1016/0003-2697(86)90244-7

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VL - 155

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EP - 187

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -

Identification of cell surface glycoproteins by periodate-alkaline phosphatase hydrazide

Abstract

Keywords

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