Identification of cell surface glycoproteins by periodate-alkaline phosphatase hydrazide

Zvi Keren, Gideon Berke, Jonathan M. Gershoni*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A novel method for the detection of cell surface glycoconjugates has been developed. Cells are subjected to mild surface oxidation of vicinal hydroxyls with sodium periodate. Afterward, cellular proteins are resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and then probed with alkaline phosphatase hydrazide. The technique is sensitive, reproducible, and inexpensive. It obviates the need for radiolabeled NaBH4 and the subsequent processing of polyacrylamide gels for fluorography. Results are easily obtained in a matter of a few hours.

Original languageEnglish
Pages (from-to)182-187
Number of pages6
JournalAnalytical Biochemistry
Volume155
Issue number1
DOIs
StatePublished - 15 May 1986
Externally publishedYes

Keywords

  • Alkaline phosphatase hydrazide
  • cell surface labeling
  • glycoproteins
  • periodate oxidation
  • protein blot

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