Identification of alpha-tropomyosin as a target self-antigen in Behçet's syndrome

Felix Mor*, Abraham Weinberger, Irun R. Cohen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Behçet's syndrome is a multi-system inflammatory disease affecting mainly the oral and urogenital mucosa and the uveal tract. The etiology and pathogenesis of Behçet's syndrome are unknown, but autoimmune mechanisms are implicated. We initiated this work to identify self-antigens targeted by patients with Behçet's syndrome. We used patient sera to immunoblot tissue lysates, and we found that some patients manifest antibodies to a 37-kDa band. The 37-kDa band was detected in extracts of skin, tongue, vagina, muscle and heart but not in brain, kidney, lung, liver, intestine and thymus. In-gel digestion and mass spectrometry revealed the band to be α-tropomyosin. Autoimmunity to α-tropomyosin can be pathogenic; immunized Lewis rats developed lesions in the uveal tract and skin, with features of Behçet's disease.

Original languageEnglish
Pages (from-to)356-365
Number of pages10
JournalEuropean Journal of Immunology
Volume32
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Autoantibody
  • Autoimmunity
  • Immunoblotting
  • Mass spectrometry
  • Pathogenesis

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