Identification of a telomere-binding activity from yeast

J Berman, C Y Tachibana, B K Tye

Research output: Contribution to journalArticlepeer-review

Abstract

In yeast, the ends of the chromosomes (telomeres) terminate in repeated poly(C1-3A) sequences. We have identified a yeast activity that binds specifically to these poly(C1-3A) repeats. An agarose gel binding assay was used to detect and characterize this activity in cell extracts using both cloned telomere DNA and yeast genomic DNA as substrates. The activity appears to bind specifically to poly(C1-3A) sequences, despite their different primary sequences, yet does not bind specifically to telomeric repeats, such as poly(C4A2), poly(C4A4), and poly (C1-8T) from other lower eukaryotes.

Original languageEnglish
Pages (from-to)3713-7
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number11
DOIs
StatePublished - Jun 1986
Externally publishedYes

Keywords

  • Base Sequence
  • Chromosomes/metabolism
  • Cloning, Molecular
  • DNA, Fungal/metabolism
  • DNA-Binding Proteins/metabolism
  • Saccharomyces cerevisiae/genetics
  • Species Specificity

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