TY - JOUR
T1 - Identification of a telomere-binding activity from yeast
AU - Berman, J
AU - Tachibana, C Y
AU - Tye, B K
PY - 1986/6
Y1 - 1986/6
N2 - In yeast, the ends of the chromosomes (telomeres) terminate in repeated poly(C1-3A) sequences. We have identified a yeast activity that binds specifically to these poly(C1-3A) repeats. An agarose gel binding assay was used to detect and characterize this activity in cell extracts using both cloned telomere DNA and yeast genomic DNA as substrates. The activity appears to bind specifically to poly(C1-3A) sequences, despite their different primary sequences, yet does not bind specifically to telomeric repeats, such as poly(C4A2), poly(C4A4), and poly (C1-8T) from other lower eukaryotes.
AB - In yeast, the ends of the chromosomes (telomeres) terminate in repeated poly(C1-3A) sequences. We have identified a yeast activity that binds specifically to these poly(C1-3A) repeats. An agarose gel binding assay was used to detect and characterize this activity in cell extracts using both cloned telomere DNA and yeast genomic DNA as substrates. The activity appears to bind specifically to poly(C1-3A) sequences, despite their different primary sequences, yet does not bind specifically to telomeric repeats, such as poly(C4A2), poly(C4A4), and poly (C1-8T) from other lower eukaryotes.
KW - Base Sequence
KW - Chromosomes/metabolism
KW - Cloning, Molecular
KW - DNA, Fungal/metabolism
KW - DNA-Binding Proteins/metabolism
KW - Saccharomyces cerevisiae/genetics
KW - Species Specificity
UR - http://www.scopus.com/inward/record.url?scp=0022506983&partnerID=8YFLogxK
U2 - 10.1073/pnas.83.11.3713
DO - 10.1073/pnas.83.11.3713
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C2 - 3520552
SN - 0027-8424
VL - 83
SP - 3713
EP - 3717
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -