Identification of a PDGF‐like mitoattractant produced by NIH/3T3 cells after transformation with SV40

Ilan Bleiberg, Anita K. Harvey, Georgeann Smale, Gary R. Grotendorst

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It has previously been shown that fibroblastic cells transformed by SV40 exhibit a reduced requirement for PDGF for growth. In addition, NIH/3T3 cells lose both their chemotactic response to PDGF and specific cell surface binding of PDGF after transformation with SV40. We have now examined whether the SV40 transformed NH/3T3 cells are producing a factor which acts similarly to PDGF. Our studies indicate that NIH/3T3 cells transformed with SV 40 produce a factor which shares many biological properties with PDGF. We were unable to detect this activity in conditioned media from non‐transformed NIH/3T3 cells. The SV40/NIH/3T3 derived factor appears to possess both chemotactic and mitogenic activity for connective tissue cells but not endothelial or epithelial cells. Furthermore, in preliminary studies, this activity competes with 125I‐PDGF for binding to smooth muscle cells. The biochemical properties of the SV40/NIH/3T3 derived factor are different from those of PDGF. The SV40 activity appears to reside in a heat labile acidic protein (pI < 7.0) of MW < 30,000 whereas PDGF is a heat stable basic protein (pI9.8) of 30,000 MW. Production of this factor may play a role in the decreased serum requirement for cell replication exhibited by SV40‐transformed NIH/3T3 cells by supplying the cells with their own PDGF‐like growth factor.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalJournal of Cellular Physiology
Issue number2
StatePublished - May 1985


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