Identification and rapid detection of three Tay-Sachs mutations in the Moroccan Jewish population

Infantile Tay-Sachs disease (TSD) is caused by mutations in the HEXA gene that result in the complete absence of β-hexosaminidase A activity. It is well known that an elevated frequency of TSD mutations exists among Ashkenazi Jews. More recently it has become apparent that elevated carrier frequencies for TSD also occur in several other ethnic groups, including Moroccan Jews, a subgroup of Sephardic Jews. Elsewhere we reported an in-frame deletion of one of the two adjacent phenylalanine codons at position 304 or 305 (ΔF(304/305)) in one HEXA allele of a Moroccan Jewish TSD patient and in three obligate carriers from six unrelated Moroccan Jewish families. We have now identified two additional mutations within exon 5 of the HEXA gene that account for the remaining TSD alleles in the patient and carriers. One of the mutations is a novel C-to-G transversion, resulting in a replacement of Tyr¹⁸⁰ by a stop codon. The other mutation is a G-to-A transition resulting in an Arg¹⁷⁰-to-Gln substitution. This mutation is at a CpG site in a Japanese infant with Tay-Sachs disease and was described elsewhere. Analysis of nine obligate carriers from seven unrelated families showed that four harbor ΔF(304/305) mutation, two the Arg¹⁷⁰→Gln mutation, and one the Tyr¹⁸⁰→Stop mutation. We also have developed rapid, nonradioactive assays for the detection of each mutation, which should be helpful for carrier screening.