TY - JOUR
T1 - Identification and mapping of N6-methyladenosine containing sequences in simian virus 40 RNA
AU - Canaani, Dan
AU - Kahana, Chaim
AU - Lavi, Sara
AU - Groner, Yoram
N1 - Funding Information:
ACKNOWLEDGEMENTS: We thank M. Revel and E. Winocour for their support and encouragemnt. A. Mukamel and Z. Grossman for excellent technical assistance. J. Sussman for aid 1n computer analysis of SV40 m6A sequences, I. Drori and P. Wynick for preparing the manuscript. This work was supported by a grant from the United States B1nat1onal Science Foundation (BSF) Jerusalem Israel. Y.G. holds the Helena Rubinstein Career Development Chair.
PY - 1979/6/25
Y1 - 1979/6/25
N2 - Late SV4O 16S and 19S mRNAs were found to contain an average of three m6A residues per mRNA molecule. The methylated residues of both the viral and cellular mRNAs occur in two sequences; Gpm6ApC and (Ap)nm6ApC where n = 1-4. More than 60% of the m6A residues in SV4O 16S and 19S mRNAs occur in Gpm6ApC even though there are twice as many (A)nAC than GAC sequences in these messengers. The m6A containing oligonucleotides of late SV40 mRNAs were localized in the viral messengers. In the 16S mRNA two m6A oligonucleotides were located at the 5′ coding region between 0.95-0.0 map units. The third m6A residue was mapped between 0.0-0.14 nap units in the translated portion of this mRNA. The overall pattern of internal methylation in the 19S mRNA is similar. However, some differences between 16S and 19S mRNAs were observed in both the content and location of the longer (Ap)nm6ApC nucleotides These results provide the first example of precise localization of internal methylation sequences in mRNA species with defined coding specificity. It implies that a) location of m6A residues is not random but specific to a particular region of the RNA, b) apart from sequence specificity other structural features of the mRNA may influence internal methylation and c) m6A residues are present in coding regions of SV40 mRNAs.
AB - Late SV4O 16S and 19S mRNAs were found to contain an average of three m6A residues per mRNA molecule. The methylated residues of both the viral and cellular mRNAs occur in two sequences; Gpm6ApC and (Ap)nm6ApC where n = 1-4. More than 60% of the m6A residues in SV4O 16S and 19S mRNAs occur in Gpm6ApC even though there are twice as many (A)nAC than GAC sequences in these messengers. The m6A containing oligonucleotides of late SV40 mRNAs were localized in the viral messengers. In the 16S mRNA two m6A oligonucleotides were located at the 5′ coding region between 0.95-0.0 map units. The third m6A residue was mapped between 0.0-0.14 nap units in the translated portion of this mRNA. The overall pattern of internal methylation in the 19S mRNA is similar. However, some differences between 16S and 19S mRNAs were observed in both the content and location of the longer (Ap)nm6ApC nucleotides These results provide the first example of precise localization of internal methylation sequences in mRNA species with defined coding specificity. It implies that a) location of m6A residues is not random but specific to a particular region of the RNA, b) apart from sequence specificity other structural features of the mRNA may influence internal methylation and c) m6A residues are present in coding regions of SV40 mRNAs.
UR - http://www.scopus.com/inward/record.url?scp=0018657266&partnerID=8YFLogxK
U2 - 10.1093/nar/6.8.2879
DO - 10.1093/nar/6.8.2879
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AN - SCOPUS:0018657266
SN - 0305-1048
VL - 6
SP - 2879
EP - 2899
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 8
ER -