Identification and mapping of N6-methyladenosine containing sequences in simian virus 40 RNA

Dan Canaani, Chaim Kahana, Sara Lavi, Yoram Groner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

152 Scopus citations

Abstract

Late SV4O 16S and 19S mRNAs were found to contain an average of three m6A residues per mRNA molecule. The methylated residues of both the viral and cellular mRNAs occur in two sequences; Gpm6ApC and (Ap)nm6ApC where n = 1-4. More than 60% of the m6A residues in SV4O 16S and 19S mRNAs occur in Gpm6ApC even though there are twice as many (A)nAC than GAC sequences in these messengers. The m6A containing oligonucleotides of late SV40 mRNAs were localized in the viral messengers. In the 16S mRNA two m6A oligonucleotides were located at the 5′ coding region between 0.95-0.0 map units. The third m6A residue was mapped between 0.0-0.14 nap units in the translated portion of this mRNA. The overall pattern of internal methylation in the 19S mRNA is similar. However, some differences between 16S and 19S mRNAs were observed in both the content and location of the longer (Ap)nm6ApC nucleotides These results provide the first example of precise localization of internal methylation sequences in mRNA species with defined coding specificity. It implies that a) location of m6A residues is not random but specific to a particular region of the RNA, b) apart from sequence specificity other structural features of the mRNA may influence internal methylation and c) m6A residues are present in coding regions of SV40 mRNAs.

Original languageEnglish
Pages (from-to)2879-2899
Number of pages21
JournalNucleic Acids Research
Volume6
Issue number8
DOIs
StatePublished - 25 Jun 1979
Externally publishedYes

Funding

FundersFunder number
United States B1nat1onal Science Foundation
United States-Israel Binational Science Foundation

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