Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris

Hermann Ehrlich; Oksana V. Kaluzhnaya; Eike Brunner; Mikhail V. Tsurkan; Alexander Ereskovsky; Micha Ilan; Konstantin R. Tabachnick; Vasilii V. Bazhenov; Silvia Paasch; Martin Kammer; René Born; Allison Stelling; Roberta Galli; Sergei Belikov; Olga V. Petrova; Victor V. Sivkov; Denis Vyalikh; Sebastian Hunoldt; Gert Wörheide

doi:10.1016/j.jsb.2013.06.015

Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris

Hermann Ehrlich^*, Oksana V. Kaluzhnaya, Eike Brunner, Mikhail V. Tsurkan, Alexander Ereskovsky, Micha Ilan, Konstantin R. Tabachnick, Vasilii V. Bazhenov, Silvia Paasch, Martin Kammer, René Born, Allison Stelling, Roberta Galli, Sergei Belikov, Olga V. Petrova, Victor V. Sivkov, Denis Vyalikh, Sebastian Hunoldt, Gert Wörheide

^*Corresponding author for this work

School of Zoology

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (¹³C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.

Original language	English
Pages (from-to)	474-483
Number of pages	10
Journal	Journal of Structural Biology
Volume	183
Issue number	3
DOIs	https://doi.org/10.1016/j.jsb.2013.06.015
State	Published - Sep 2013

Funding

Funders	Funder number
Erasmus Mundus DAAD
German-Russian Interdisciplinary Science Center
Deutsche Forschungsgemeinschaft	BR 1278/ 17-1, EH 394/1-1
Russian Foundation for Basic Research	11-04-00323-а
Bundesministerium für Bildung und Forschung	03EK3029A

Keywords

Bioanalytics
Chitin
Chitin synthase
Skeleton
Sponges

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10.1016/j.jsb.2013.06.015

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Ehrlich, H., Kaluzhnaya, O. V., Brunner, E., Tsurkan, M. V., Ereskovsky, A., Ilan, M., Tabachnick, K. R., Bazhenov, V. V., Paasch, S., Kammer, M., Born, R., Stelling, A., Galli, R., Belikov, S., Petrova, O. V., Sivkov, V. V., Vyalikh, D., Hunoldt, S., & Wörheide, G. (2013). Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris. Journal of Structural Biology, 183(3), 474-483. https://doi.org/10.1016/j.jsb.2013.06.015

@article{c264d5f33d45410eb2ccf2154aea06ac,

title = "Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris",

abstract = "This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.",

keywords = "Bioanalytics, Chitin, Chitin synthase, Skeleton, Sponges",

author = "Hermann Ehrlich and Kaluzhnaya, {Oksana V.} and Eike Brunner and Tsurkan, {Mikhail V.} and Alexander Ereskovsky and Micha Ilan and Tabachnick, {Konstantin R.} and Bazhenov, {Vasilii V.} and Silvia Paasch and Martin Kammer and Ren{\'e} Born and Allison Stelling and Roberta Galli and Sergei Belikov and Petrova, {Olga V.} and Sivkov, {Victor V.} and Denis Vyalikh and Sebastian Hunoldt and Gert W{\"o}rheide",

note = "Funding Information: Financial support by DFG (projects EH 394/1-1 and BR 1278/ 17-1) is gratefully acknowledged. Furthermore, this study was funded by Erasmus Mundus DAAD Program 2011, RFBR No. 11-04-00323-а, G-RISC (German-Russian Interdisciplinary Science Center) Grant 2012, the BMBF within the project CryPhysConcept (03EK3029A) and BHMZ Programme of Dr.-Erich-Kr{\"u}ger- Foundation (Germany). ",

year = "2013",

month = sep,

doi = "10.1016/j.jsb.2013.06.015",

language = "אנגלית",

volume = "183",

pages = "474--483",

journal = "Journal of Structural Biology",

issn = "1047-8477",

publisher = "Academic Press Inc.",

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Ehrlich, H, Kaluzhnaya, OV, Brunner, E, Tsurkan, MV, Ereskovsky, A, Ilan, M, Tabachnick, KR, Bazhenov, VV, Paasch, S, Kammer, M, Born, R, Stelling, A, Galli, R, Belikov, S, Petrova, OV, Sivkov, VV, Vyalikh, D, Hunoldt, S & Wörheide, G 2013, 'Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris', Journal of Structural Biology, vol. 183, no. 3, pp. 474-483. https://doi.org/10.1016/j.jsb.2013.06.015

TY - JOUR

T1 - Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris

AU - Ehrlich, Hermann

AU - Kaluzhnaya, Oksana V.

AU - Brunner, Eike

AU - Tsurkan, Mikhail V.

AU - Ereskovsky, Alexander

AU - Ilan, Micha

AU - Tabachnick, Konstantin R.

AU - Bazhenov, Vasilii V.

AU - Paasch, Silvia

AU - Kammer, Martin

AU - Born, René

AU - Stelling, Allison

AU - Galli, Roberta

AU - Belikov, Sergei

AU - Petrova, Olga V.

AU - Sivkov, Victor V.

AU - Vyalikh, Denis

AU - Hunoldt, Sebastian

AU - Wörheide, Gert

N1 - Funding Information: Financial support by DFG (projects EH 394/1-1 and BR 1278/ 17-1) is gratefully acknowledged. Furthermore, this study was funded by Erasmus Mundus DAAD Program 2011, RFBR No. 11-04-00323-а, G-RISC (German-Russian Interdisciplinary Science Center) Grant 2012, the BMBF within the project CryPhysConcept (03EK3029A) and BHMZ Programme of Dr.-Erich-Krüger- Foundation (Germany).

PY - 2013/9

Y1 - 2013/9

N2 - This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.

AB - This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (13C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor.

KW - Bioanalytics

KW - Chitin

KW - Chitin synthase

KW - Skeleton

KW - Sponges

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U2 - 10.1016/j.jsb.2013.06.015

DO - 10.1016/j.jsb.2013.06.015

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AN - SCOPUS:84883466136

SN - 1047-8477

VL - 183

SP - 474

EP - 483

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 3

ER -

Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris

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Keywords

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