Hyperreflective Foci Along the Retinal Pigment Epithelium Predict the Onset of Large Choroidal Hypertransmission Defects in Age-Related Macular Degeneration

Alessandro Berni, James D. Kastner, Mengxi Shen, Yuxuan Cheng, Gissel Herrera, Farhan Hiya, Jeremy Liu, Liang Wang, Jianqing Li, Omar S. El-Mulki, Sara Beqiri, Omer Trivizki, Nadia K. Waheed, Robert O'Brien, Giovanni Gregori, Ruikang K. Wang, Philip J. Rosenfeld*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: In eyes with intermediate age-related macular degeneration (iAMD), we separately quantified the hyperreflective foci (HRF) along the retinal pigment epithelium (rpeHRF) and the intraretinal HRF (iHRF) to determine if the location of the HRF predicted the progression from iAMD to the onset of large persistent choroidal hypertransmission defects (hyperTDs). Design: Post hoc subgroup cohort analysis of a prospective study. Methods: A retrospective analysis was performed on a prospective natural history database of eyes with AMD imaged using swept-source optical coherence tomography (SS-OCT). En face images derived from choroidal slabs positioned 64 to 400 µm beneath Bruch membrane were used with a semiautomated algorithm to identify and quantify hypotransmission defects (hypoTDs) attributable to either iHRF or rpeHRF within a 5-mm fovea-centered circle. iHRF were identified on corresponding B-scans as hyperreflective lesions within the neurosensory retina, and rpeHRF were identified as areas of retinal pigment epithelium thickening. Multivariable survival analysis was performed to determine if the area measurements of either iHRF or rpeHRF were more likely to predict the onset of the first large persistent hyperTD. Results: Of the 171 eyes with iAMD included in this study, 82 (48%) developed at least 1 large hyperTD during a median follow-up of 59.1 months. Univariable Cox regression analyses showed that rpeHRF area (P < .001), iHRF area (P = .003), and drusen volume (P < .001) were all significantly associated with the onset of the first large persistent hyperTD. However, a multivariable Cox regression model showed that only the rpeHRF area remained a significant predictor of disease progression (P < .001). Conclusions: In iAMD eyes, the area of rpeHRF was more predictive of disease progression than either the drusen volume or iHRF, which suggests that these rpeHRF serve as harbingers of focal atrophy formation and may predict where hyperTDs form.

Original languageEnglish
Pages (from-to)76-90
Number of pages15
JournalAmerican Journal of Ophthalmology
Volume274
DOIs
StatePublished - Jun 2025

Funding

FundersFunder number
Research to Prevent Blindness
Carl Zeiss Meditec AG
Colgate-Palmolive
Gyroscope Therapeutics
National Eye InstituteR01 EY028753, P30EY014801

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