TY - JOUR
T1 - Hydrogel-integrated 3D-printed poly(lactic acid) scaffolds for bone tissue engineering
AU - Das, Mitun
AU - Sharabani-Yosef, Orna
AU - Eliaz, Noam
AU - Mandler, Daniel
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to The Materials Research Society.
PY - 2021/10/14
Y1 - 2021/10/14
N2 - Abstract: There is currently a high demand for synthetic biodegradable scaffolds with enhanced osteogenic and angiogenic performance for the regeneration of large-size bone defects. Here, hybrid scaffolds were prepared by integrating either alginate or alginate–bioglass composite hydrogels with a 3D-printed poly(lactic acid) (PLA) porous structure. The as-deposited PLA scaffolds were surface treated with polyacrylic acid (PAA), which significantly enhanced the PLA scaffold’s wettability. The surface-modified PLA scaffolds integrated well with hydrogels and provided shape and mechanical rigidity to the hydrogel. In phosphate-buffered saline, the lowest weight loss during 21-days immersion was measured for the PLA scaffold, while alginate–bioglass scaffolds lost ~ 1.9% weight during the first 7 days of immersion. In vitro cytocompatibility tests indicated good cell viability and cell proliferation on the scaffolds. The bioglass-containing hybrid scaffold promoted osteogenic differentiation and calcium mineralization. The excellent biocompatibility, good mechanical stability of the hydrogel, and shape retention of the novel hybrid scaffolds with cell-laden alginate could make them attractive for large bone regeneration. Graphic abstract: [Figure not available: see fulltext.]
AB - Abstract: There is currently a high demand for synthetic biodegradable scaffolds with enhanced osteogenic and angiogenic performance for the regeneration of large-size bone defects. Here, hybrid scaffolds were prepared by integrating either alginate or alginate–bioglass composite hydrogels with a 3D-printed poly(lactic acid) (PLA) porous structure. The as-deposited PLA scaffolds were surface treated with polyacrylic acid (PAA), which significantly enhanced the PLA scaffold’s wettability. The surface-modified PLA scaffolds integrated well with hydrogels and provided shape and mechanical rigidity to the hydrogel. In phosphate-buffered saline, the lowest weight loss during 21-days immersion was measured for the PLA scaffold, while alginate–bioglass scaffolds lost ~ 1.9% weight during the first 7 days of immersion. In vitro cytocompatibility tests indicated good cell viability and cell proliferation on the scaffolds. The bioglass-containing hybrid scaffold promoted osteogenic differentiation and calcium mineralization. The excellent biocompatibility, good mechanical stability of the hydrogel, and shape retention of the novel hybrid scaffolds with cell-laden alginate could make them attractive for large bone regeneration. Graphic abstract: [Figure not available: see fulltext.]
KW - Additive manufacturing
KW - Alginate–bioglass hydrogel
KW - Biomedical
KW - Bone
KW - In vitro cell culture
KW - Poly(lactic acid) (PLA)
UR - http://www.scopus.com/inward/record.url?scp=85119233917&partnerID=8YFLogxK
U2 - 10.1557/s43578-021-00201-w
DO - 10.1557/s43578-021-00201-w
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AN - SCOPUS:85119233917
SN - 0884-2914
VL - 36
SP - 3833
EP - 3842
JO - Journal of Materials Research
JF - Journal of Materials Research
IS - 19
ER -