@article{c3b67ae41d1f49589a6f0f736223fce9,
title = "Hybridoma Antibodies to the Lipid-Binding Site(S) in the Amino-Terminal Region of Fibronectin Inhibits Binding of Streptococcal Lipoteichoic Acid",
abstract = "In this report, we present evidence to suggest that streptococci and lipoteichoic acid (LTA) interact with a fatty acid binding site located near the NH2-terminus of fibronectin. The evidence is based on the following observations. (1) Antibodies directed against a synthetic peptide (residues 1–30 of the amino-terminus of fibronectin) reacted with the two thermolysin-generated peptides (24 and 28 kilodaltons [kDa)) that were adsorbed by and eluted from streptococci. (2) The adsorption of the 24- and 28-kDa peptides to streptococci was inhibited by LTA. (3) The two monoclonal antibodies that inhibited the binding of LTA to fibronectin reacted only with the 24- and 28-kDA fragments of fibronectin. (4) Conversely, LTA, as well as lauric acid and oleic acid, blocked the binding of the same monoclonal antibodies to fibronectin. (5) LTA had no effect on the binding of hybridoma antibodies directed against the collagen or cell-binding domain.",
author = "Lena Stanislawski and Courtney, {Harry S.} and Simpson, {W. Andrew} and Hasty, {David L.} and Beachey, {Edwin H.} and L. Robert and Itzhak Ofek",
note = "Funding Information: Received for publication 23 December 1986,and in revised form 16 March 1987. This work was supported by research funds CNRS (UA1l71) from the Fondation pour la Recherche Medicale Francaise and the Conseil Scientifique of the Universite Paris Val de Marne; by the U. S. Veterans Administration; and by grants AI-l0085 and AI-13550 from the National Institute of Allergy and Infectious Diseases, DE-07218 from the National Institute of Dental Research, and HL-26009 from the National Heart, Lung, and Blood Institute. We thank Johnnie Smith for secretarial assistance. Please address requests for reprints to Dr. Edwin Beachey, VeteransAdministrationMedicalCenter(IllM), 1030JeffersonAvenue, Memphis, Tennessee 38104.",
year = "1987",
month = aug,
doi = "10.1093/infdis/156.2.344",
language = "אנגלית",
volume = "156",
pages = "344--349",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "2",
}