Human papillomavirus types detected in skin warts and cancer differ in their transforming properties but commonly counteract UVB induced protective responses in human keratinocytes

Naama Shterzer, Dariya Heyman, Beny Shapiro, Abraham Yaniv, Anna Jackman, Francis Serour, Malka Chaouat, Pinhas Gonen, Massimo Tommasino, Levana Sherman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In the present study, E6E7 and E6 proteins of human papillomaviruses (HPVs) associated with skin warts and cancer were compared for their transforming and carcinogenic abilities in primary human keratinocytes (PHKs). We show that E6E7 of cancer associated beta HPV types, notably 49 and 24, were able to extend the life span and enhance the clonogenic efficiency of PHKs when maintained in serum free/low calcium medium. Activities of the beta HPV E6E7 were lower than those of HPV16 E6E7. In contrast, E6 proteins from HPV types detected in skin warts or cancer, notably 10, 49 and 38, attenuated UVB induced protective responses in PHKs including cell death, proliferation arrest and accumulation of the proapoptotic proteins, p53, bax or bak. Together, this investigation revealed functional differences and commonalities between HPVs associated with skin warts and cancer, and allowed the identification of specific properties of beta HPVs supporting their involvement in skin carcinogenesis.

Original languageEnglish
Pages (from-to)647-659
Number of pages13
JournalVirology
Volume468
DOIs
StatePublished - 1 Nov 2014

Funding

FundersFunder number
Ministry of Justice, Israel
Israel Cancer Association USA3-7121

    Keywords

    • Apoptosis
    • Bak
    • Bax
    • Beta HPVs
    • Cutaneous HPV types
    • Skin
    • Skin cancer
    • UVB
    • Warts
    • p53

    Fingerprint

    Dive into the research topics of 'Human papillomavirus types detected in skin warts and cancer differ in their transforming properties but commonly counteract UVB induced protective responses in human keratinocytes'. Together they form a unique fingerprint.

    Cite this