Human mast cells release metalloproteinase-9 on contact with activated T cells: Juxtacrine regulation by TNF-α

D. Baram, G. G. Vaday, P. Salamon, I. Drucker, R. Hershkoviz, Y. A. Mekori

Research output: Contribution to journalArticlepeer-review

Abstract

Mast cells, essential effector cells in allergic inflammation, have been found to be activated in T cell-mediated inflammatory processes in accordance with their residence in close physical proximity to T cells. We have recently reported that mast cells release granule-associated mediators and TNF-α upon direct contact with activated T cells. This data suggested an unrecognized activation pathway, where mast cells may be activated during T cell-mediated inflammation. Herein, we show that this cell-cell contact results in the release of matrix metalloproteinase (MMP)-9 and the MMP inhibitor tissue inhibitor of metalloproteinase 1 from HMC-I human mast cells or from mature peripheral blood-derived human mast cells. The expression and release of these mediators, as well as of β-hexosaminidase and several cytokines, were also induced when mast cells were incubated with cell membranes isolated from activated, but not resting, T cells. Subcellular fractionation revealed that the mature form of MMP-9 cofractionated with histamine and tryptase, indicating its localization within the secretory granules. MMP-9 release was first detected at 6 h and peaked at 22 h of incubation with activated T cell membranes, while TNF-α release peaked after only 6 h. Anti-TNF-α mAb inhibited the T cell membrane-induced MMP-9 release, indicating a possible autocrine regulation of MMP release by mast cell TNF-α. This cascade of events, whereby mast cells are activated by T cells to release cytokines and MMP-9, which are known to be essential for leukocyte extravasation and recruitment to affected sites, points to an important immunoregulatory function of mast cells within the context of T cell-mediated inflammatory processes.

Original languageEnglish
Pages (from-to)4008-4016
Number of pages9
JournalJournal of Immunology
Volume167
Issue number7
DOIs
StatePublished - 1 Oct 2001
Externally publishedYes

Fingerprint

Dive into the research topics of 'Human mast cells release metalloproteinase-9 on contact with activated T cells: Juxtacrine regulation by TNF-α'. Together they form a unique fingerprint.

Cite this