Human lymphocyte dependent antibody mediated cytotoxicity: Adherence of lymphocytes to antibody treated target cells

I. Yust, R. W. Smith, H. B. Dickler, J. Wunderlich, D. L. Mann

Research output: Contribution to journalArticlepeer-review

Abstract

An in vitro human antibody dependent cell mediated cytotoxicity system was used to study the adherence of nonsensitized attacking lymphocytes from peripheral blood to antibody coated melanoma target cells. Specific adherence of attacking cells was documented by labeling the lymphocytes with 51Cr. The degree of specific adherence was proportional to antibody concentration and incubation time and could be detected before the lysis of target cells. Adsorption of attacking lymphocytes on immune serum treated target cells depleted B cells, enriched T cells, and removed most cytotoxic activity of nonadherent lymphocytes in this system. These results were not found when attacking lymphocytes were adsorbed on normal serum treated target cells.

Original languageEnglish
Pages (from-to)176-186
Number of pages11
JournalCellular Immunology
Volume18
Issue number1
DOIs
StatePublished - Jul 1975
Externally publishedYes

Fingerprint

Dive into the research topics of 'Human lymphocyte dependent antibody mediated cytotoxicity: Adherence of lymphocytes to antibody treated target cells'. Together they form a unique fingerprint.

Cite this