Human lupus monoclonal autoantibodies bind to Raji cells

Yehuda Shoenfeld*, Nechama I. Smorodinsky, Gad Lavie, Bilha Hazaz, Henri Joshua, Jacob Pinkhas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Seven of 60 human monoclonal anti-DNA autoantibodies derived from 3 patients with SLE were shown to bind to Raji cells by radioimmunoassay. The binding of the lupus autoantibodies to the Raji cells in solution was not affected by prior incubation of the cells with DNase. Preincubation of the monoclonal autoantibodies with polynucleotides and cardiolipin, resulted in significant inhibition which correlated with the direct binding characteristics of these antibodies. Previous results with mouse IgG monoclonal anti-DNA antibodies supported by our results with human IgM anti-DNA autoantibodies suggest caution in interpreting analyses of immune complexes of sera containing anti-DNA antibodies entailing Raji cells.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalImmunology Letters
Volume11
Issue number2
DOIs
StatePublished - 1985

Keywords

  • Raji cells
  • human lupus monoclonal autoantibodies

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