Human long bone development in vivo: Analysis of the distal femoral epimetaphysis on mr images of fetuses

Ursula Nemec, Stefan F. Nemec*, Michael Weber, Peter C. Brugger, Gregor Kasprian, Dieter Bettelheim, David L. Rimoin, Ralph S. Lachman, Gustavo Malinger, Daniela Prayer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Purpose: To investigate human long bone development in vivo by analyzing distal femoral epimetaphyseal structures and bone morphometrics on magnetic resonance (MR) images of fetuses. Materials and Methods: An institutional review board approved this retrospective study, and informed consent was waived. Included were 272 MR imaging examinations (April 2004-July 2011) in 253 fetuses with a mean gestational age (GA) of 26 weeks±days (range, 19 weeks 2 days to 35 weeks±days) without known musculoskeletal abnormalities. Two independent readers qualitatively analyzed epiphyseal and metaphyseal shape, secondary ossification, and the perichondrium on 1.5-T echo-planar MR images and correlated the results with the GA that was derived from previous fetal ultrasonography (US). Diaphyseal and epiphyseal morphometric measurements were correlated with GA by means of the Pearson correlation and linear regression. MR imaging measurements of diaphyseal length and US normative values were compared graphically. Interreader agreement analysis was performed with weighted k statistics and the intraclass correlation coefficient. Results: With advancing GA, the epiphyseal shape changed from spherical (r2 = 0.664) to hemispherical with a notch (r2 = 0.804), and the metaphyseal shape changed from flat (r2 = 0.766) to clearly undulated (r2 = 0.669). Secondary ossification (r2 = 0.777) was not observed until 25 weeks 3 days. The perichondrium decreased (r2 = 0.684) from 20 weeks onward. Correlation coefficients were 0.897 for diaphyseal length, 0.738 for epiphyseal length, and 0.801 for epiphyseal width with respect to GA. The range of measurements of diaphyseal length was larger than that of the reported US normative values. Interreader agreement was good for bone morphometrics (intraclass correlation coefficient, 0.906-0.976), and moderate for bone characteristics (weighted k, 0.448-0.848). Conclusion: Prenatal MR imaging allows visualization of human bone development in vivo by means of epimetaphyseal characteristics and bone morphometrics.

Original languageEnglish
Pages (from-to)570-580
Number of pages11
JournalRadiology
Volume267
Issue number2
DOIs
StatePublished - May 2013
Externally publishedYes

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