TY - JOUR
T1 - Human follicular nitric oxide pathway
T2 - Relationship to follicular size, oestradiol concentrations and ovarian blood flow
AU - Anteby, Eyal Y.
AU - Hurwitz, Arye
AU - Korach, Osnat
AU - Revel, Ariel
AU - Simon, Alex
AU - Finci-Yeheskel, Zvezdana
AU - Mayer, Michael
AU - Laufer, Neri
PY - 1996/9
Y1 - 1996/9
N2 - Nitric oxide (NO) has been suggested to be involved in ovarian physiology. Our aim was to study follicular nitrite and nitrate (NO3/NO2) levels in women undergoing in-vitro fertilization (IVF), and to examine their relationship to follicular size, oestradiol concentrations, and ovarian artery and intra-ovarian blood flow as measured by Doppler ultrasound. A total of 15 patients from the IVF programme of Hadassah University Hospital, Mt Scopus, Israel, participated in the study. Detailed transvaginal ultrasonographic examination was performed before ovum collection, and ovarian artery and intra-ovarian blood flow were measured. While aspirating the follicles, the content of two to four of the follicles in each ovary was collected individually, the volume of follicular fluid measured, and NO3/NO2 concentrations were determined. A statistically significant positive correlation was found between follicular fluid NO3/NO2 concentrations and follicular volume (r = 0.76), as well as between NO3/NO2 concentrations and oestradiol concentrations (r = 0.63). A statistically significant negative correlation was found between follicular fluid NO3/NO2 concentrations and ovarian flow parameters as well as between NO3/NO2 concentrations in follicles containing 4-5 ml and ovarian artery pulsatility index.
AB - Nitric oxide (NO) has been suggested to be involved in ovarian physiology. Our aim was to study follicular nitrite and nitrate (NO3/NO2) levels in women undergoing in-vitro fertilization (IVF), and to examine their relationship to follicular size, oestradiol concentrations, and ovarian artery and intra-ovarian blood flow as measured by Doppler ultrasound. A total of 15 patients from the IVF programme of Hadassah University Hospital, Mt Scopus, Israel, participated in the study. Detailed transvaginal ultrasonographic examination was performed before ovum collection, and ovarian artery and intra-ovarian blood flow were measured. While aspirating the follicles, the content of two to four of the follicles in each ovary was collected individually, the volume of follicular fluid measured, and NO3/NO2 concentrations were determined. A statistically significant positive correlation was found between follicular fluid NO3/NO2 concentrations and follicular volume (r = 0.76), as well as between NO3/NO2 concentrations and oestradiol concentrations (r = 0.63). A statistically significant negative correlation was found between follicular fluid NO3/NO2 concentrations and ovarian flow parameters as well as between NO3/NO2 concentrations in follicles containing 4-5 ml and ovarian artery pulsatility index.
KW - Blood flow
KW - Nitric oxide
KW - Nitrite:nitrate
KW - Oestradiol
KW - Ovarian follicle
UR - http://www.scopus.com/inward/record.url?scp=0029800487&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.humrep.a019522
DO - 10.1093/oxfordjournals.humrep.a019522
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C2 - 8921069
AN - SCOPUS:0029800487
VL - 11
SP - 1947
EP - 1951
JO - Human Reproduction
JF - Human Reproduction
SN - 0268-1161
IS - 9
ER -