Human embryonic stem cell transplantation to repair the infarcted myocardium

Jonathan Leor*, Sharon Gerecht, Smadar Cohen, Liron Miller, Radka Holbova, Anna Ziskind, Michal Shachar, Micha S. Feinberg, Esther Guetta, Joseph Itskovitz-Eldor

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Objective: To test the hypothesis that human embryonic stem cells (hESCs) can be guided to form new myocardium by transplantation into the normal or infarcted heart, and to assess the influence of hESC-derived cardiomyocytes (hESCMs) on cardiac function in a rat model of myocardial infarction (MI). Methods: Undifferentiated hESCs (0.5-1 × 106), human embryoid bodies (hEBs) (4-8 days; 0.5-1 × 106), 0.1 mm pieces of embryonic stem-derived beating myocardial tissue, and phosphate-buffered saline (control) were injected into the normal or infarcted myocardium of athymic nude rats (n = 58) by direct injection into the muscle or into preimplanted three-dimensional alginate scaffold. By 2-4 weeks after transplantation, heart sections were examined to detect the human cells and differentiation with fluorescent in situ hybridisation, using DNA probes specific for human sex chromosomes and HLA-DR or HLA-ABC immunostaining. Results: Microscopic examination showed transplanted human cells in the normal, and to a lesser extent in the infarcted myocardium (7/7 vs 2/6; p<0.05). The transplanted hESCs and hEBs rarely created new vessels and did not form new myocardium. Transplantation of hESCM tissue into normal heart produced islands of disorganised myofibres, fibrosis and, in a single case, a teratoma. However, transplantation of hESCMs into the infarcted myocardium did prevent post-MI dysfunction and scar thinning. Conclusions: Undifferentiated hESCs and hEBs are not directed to form new myocardium after transplantation into normal or infarcted heart and may create teratoma. Nevertheless, this study shows that hESC-derived cardiomyocyte transplantation can attenuate post-MI scar thinning and left ventricular dysfunction.

Original languageEnglish
Pages (from-to)1278-1284
Number of pages7
Issue number10
StatePublished - Oct 2007


Dive into the research topics of 'Human embryonic stem cell transplantation to repair the infarcted myocardium'. Together they form a unique fingerprint.

Cite this