TY - JOUR
T1 - Human Cu/Zn superoxide dismutase gene
T2 - Molecular characterization of its two mRNA species
AU - Sherman, Levana
AU - Levanon, Ditsa
AU - Lieman-Hurwitz, Judy
AU - Dafni, Naomi
AU - Groner, Yoram
N1 - Funding Information:
ACKNOWLEDGEMENTS This work was supported by the U.S.-Israel Binational Science Foundation, Jerusalem, Israel, by the Biotechnology General Corp., Israel, and by the Leo and Julia Forschheimer Center for Molecular Genetics. L.S. is a fellow of the Sir Charles Clore Memorial Fund.
PY - 1984/12/21
Y1 - 1984/12/21
N2 - Two cytoplasmic superoxide dismutase (SOD-1) mRNAs of about 0.7 and 0.9 kilobases (Kb.) were previously found in a variety of human cells. The two SOD-1 mRNAs are transcribed from the same gene and the major 0.7 Kb. species is approximately four times more abundant than the minor 0.9 Kb. mRNA. These two mRNAs differ in the length of their 3′-untranslated region and both have multiple 5′-ends. The longer transcript contains 222 additional nucleotides beyond the 3′-polyadenylated terminus of the short mRNA. S1 nuclease mapping and sequence analysis showed that these extra 222 nucleotides are specified by sequences contiguous to those shared by the two SOD-1 mRNAs. The 5′-termini of the two SOD-1 mRNAs were identified and mapped by both primer extension and S1 mapping. The majority of SOD-1 mRNA molecules (90-95%) have a 5′-start site located 23 base pairs (b.p.) downstream of the hexanucleotide -TATAAA-. The rest of the SOD-1 mRNA molecules have 5′-termini 30, 50 and 65 b.p. upstream from the major start region.
AB - Two cytoplasmic superoxide dismutase (SOD-1) mRNAs of about 0.7 and 0.9 kilobases (Kb.) were previously found in a variety of human cells. The two SOD-1 mRNAs are transcribed from the same gene and the major 0.7 Kb. species is approximately four times more abundant than the minor 0.9 Kb. mRNA. These two mRNAs differ in the length of their 3′-untranslated region and both have multiple 5′-ends. The longer transcript contains 222 additional nucleotides beyond the 3′-polyadenylated terminus of the short mRNA. S1 nuclease mapping and sequence analysis showed that these extra 222 nucleotides are specified by sequences contiguous to those shared by the two SOD-1 mRNAs. The 5′-termini of the two SOD-1 mRNAs were identified and mapped by both primer extension and S1 mapping. The majority of SOD-1 mRNA molecules (90-95%) have a 5′-start site located 23 base pairs (b.p.) downstream of the hexanucleotide -TATAAA-. The rest of the SOD-1 mRNA molecules have 5′-termini 30, 50 and 65 b.p. upstream from the major start region.
UR - http://www.scopus.com/inward/record.url?scp=0021768595&partnerID=8YFLogxK
U2 - 10.1093/nar/12.24.9349
DO - 10.1093/nar/12.24.9349
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0021768595
SN - 0305-1048
VL - 12
SP - 9349
EP - 9365
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 24
ER -