TY - JOUR
T1 - HO-1 and VEGF gene expressions are time dependant during exposure to welding fumes
AU - Stark, M.
AU - Zubareb, J.
AU - Jacovovitz, R.
AU - Schwartz, Y.
AU - Lerman, Y.
AU - Grinberg, N.
AU - Fireman, E.
PY - 2009/5
Y1 - 2009/5
N2 - Hemeoxygenase-1 (HO-1) is a defensive enzyme against oxidative stress. Vascular epithelial growth factor (VEGF) is a potent cytokine which promotes angiogenesis. We used induced sputum (IS) technology to study HO-1 and VEGF expressions in neutrophilic inflammation in asymptomatic welders. Aircraft plant employees were divided into three groups: Welders 1 (n = 30) had short-term exposure to aluminum/iron, Welders 2 (n = 16) had long-term exposure to cadmium/chromium/iron/nickel, and controls (n = 27 non-exposed individuals). Participants underwent pulmonary function tests (PFTs), IS, differential cell counts, and determination of particle size distribution in IS samples. HO-1 and VEGF gene expressions were analyzed by real-time polymerase chain reaction, and protein levels were measured by bilirubin reductase-dependant reaction and ELISA, respectively. All subjects had normal PFTs. Welders 2 had neutrophilic inflammation and higher percentages of particles between 2-5 μ than the other groups. HO-1 inversely correlated with VEGF gene expression: HO-1 was significantly higher and VEGF was significantly lower in the Welders 1 group than in the other groups. There was a correlation between HO-1 expression and protein activity (r = 0.33, P = 0.05). Particulate matters significantly influenced HO-1 and VEGF gene expressions, caused neutrophilic inflammation and promoted oxidative stress in welders with long-term exposure.
AB - Hemeoxygenase-1 (HO-1) is a defensive enzyme against oxidative stress. Vascular epithelial growth factor (VEGF) is a potent cytokine which promotes angiogenesis. We used induced sputum (IS) technology to study HO-1 and VEGF expressions in neutrophilic inflammation in asymptomatic welders. Aircraft plant employees were divided into three groups: Welders 1 (n = 30) had short-term exposure to aluminum/iron, Welders 2 (n = 16) had long-term exposure to cadmium/chromium/iron/nickel, and controls (n = 27 non-exposed individuals). Participants underwent pulmonary function tests (PFTs), IS, differential cell counts, and determination of particle size distribution in IS samples. HO-1 and VEGF gene expressions were analyzed by real-time polymerase chain reaction, and protein levels were measured by bilirubin reductase-dependant reaction and ELISA, respectively. All subjects had normal PFTs. Welders 2 had neutrophilic inflammation and higher percentages of particles between 2-5 μ than the other groups. HO-1 inversely correlated with VEGF gene expression: HO-1 was significantly higher and VEGF was significantly lower in the Welders 1 group than in the other groups. There was a correlation between HO-1 expression and protein activity (r = 0.33, P = 0.05). Particulate matters significantly influenced HO-1 and VEGF gene expressions, caused neutrophilic inflammation and promoted oxidative stress in welders with long-term exposure.
KW - Induced sputum
KW - Oxidative stress
KW - Welders
UR - http://www.scopus.com/inward/record.url?scp=67349194004&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2009.02.012
DO - 10.1016/j.cyto.2009.02.012
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C2 - 19303319
AN - SCOPUS:67349194004
SN - 1043-4666
VL - 46
SP - 290
EP - 295
JO - Cytokine
JF - Cytokine
IS - 2
ER -