HLA-G expression is induced in Epstein-Barr virus-transformed B-cell lines by culture conditions

Ephraim Gazit*, Maya Sherf, Elkana Balbin, Anna Muratov, Itamar Goldstein, Ron Loewenthal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Human leukocyte antigen-G (HLA-G) belongs to the nonclassical HLA class I family of genes presently designated as class Ib genes. It was found to be expressed mainly in placental tissue and in the thymus. Expression of HLA-G is induced by lymphokines such as interleukin-10 and has been associated with the escape of tumor cells from immune surveillance or with inhibition of graft rejection. In this report, Epstein-Barr virus-transformed B-cell lines established from peripheral blood lymphocytes from healthy volunteers were studied. Our results show that EBV-transformed B-cell lines, but not freshly separated peripheral blood lymphocytes, can be induced to express HLA-G either by subjecting the cultures to nutrient deficiency to hypoxia or to both, however, not all cell lines responded equally to stress conditions. The association of HLA-G with certain cancer transformations may suggest that the resistance to HLA-G expression could be related to susceptibility to the development of malignancy.

Original languageEnglish
Pages (from-to)463-468
Number of pages6
JournalHuman Immunology
Issue number6
StatePublished - Jun 2007
Externally publishedYes


  • B-cell lines
  • Epstein-Barr virus
  • HLA-G
  • Tissue culture


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