Histopathological changes of trabecular meshwork in goniodysgenetic glaucoma in rabbits

A. Ueno, A. Tawara, T. Kubota, H. Inomata, A. S. Solomon

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Purpose. In previous histologic studies of the chamber angle in goniodysgenetic glaucoma, we observed a thick subcanalicular tissue with a structure similar to the endothelial meshwork beneath the inner wall of Schlemm's canal. Another characteristic finding was that the trabecular meshwork contains a great amount of extracelluler matrix of basal lamina-like materials and fine fibrillar materials. The present study was performed to study histologically the fine structure of trabecular meshwork in rabbits with congenital glaucoma Methods. Two congenital glaucoma rabbits, genetically developed for research purposes, were used for this study. The enucleated eyes were fixed with 4% glutaraldehyde in cacodylate buffer (pH 7.6) and osmium tetraoxide in the same buffer. The trabecular tissues were then embedded in Epon. Semi-thin sections were stained with Azure II for light microscopy. Ultra-thin section stained with uranil acetate and lead citrate were observed with an electron microscope. Results. The trabecular tissues in glaucoma eyes showed no differneces in both animals. The angular aqueous plexus, which corresponds to Schlemm's canal in humans, was observed to open far behind of the posterior portion of the the iridocorneal angle. In the angular meshwork, which corresponds to the trabecular meshwork in humans, round-formed cells were found covering the extracellular matrix. A large amount of extracellular matrix of basal lamina-like material was observed in the angular meshwork. In other eyes with normal intraocular pressue, trabecular beams were covered with slender trabecular cells. Conclusions. These congenital glaucoma rabbits histopathologically seem to have a similarity with a goniodysgenetic glaucoma in humans. This rabbit might have a great potential advantages identical to goniodysgenrtic glaucoma.

Original languageEnglish
Pages (from-to)S823
JournalInvestigative Ophthalmology and Visual Science
Issue number3
StatePublished - 15 Feb 1996


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