Histone H4 acetylation and AZFc involvement in germ cells of specimens of impaired spermatogenesis

Sandra E. Kleiman; Batia Bar-Shira Maymon; Ron Hauser; Amnon Botchan; Gedalia Paz; Haim Yavetz; Leah Yogev

doi:10.1016/j.fertnstert.2007.05.068

Histone H4 acetylation and AZFc involvement in germ cells of specimens of impaired spermatogenesis

Sandra E. Kleiman^*, Batia Bar-Shira Maymon, Ron Hauser, Amnon Botchan, Gedalia Paz, Haim Yavetz, Leah Yogev

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To measure histone-H4 acetylation and involvement of the AZFc region in testicular mixed atrophy. Design: Prospective study. Setting: University-affiliated medical center. Patient(s): Azoospermic men (n = 23) who underwent testicular sperm extraction and preparation for intracytoplasmic sperm injection (ICSI) divided into obstructive azoospermia with complete spermatogenesis (group A), testicular mixed atrophy (group B), and testicular mixed atrophy associated with AZFc deletion (group C). Intervention(s): Testicular biopsy evaluation by Western blotting and quantitative immunohistochemistry of histone-H4 hyperacetylation (Hypac-H4) and lysine-12 acetylation (Lys12ac-H4). Main Outcome Measure(s): Percentage of spermatogonia and spermatids stained by Hypac-H4 and Lys12ac-H4 antibodies in retrieved specimens. Result(s): The percentage of spermatogonia stained for Hypac-H4 and Lys12ac-H4 in groups B and C was statistically significantly reduced. The percentage of elongated spermatids showing positive staining to Hypac-H4 was statistically significantly lower in group B than group A. The percentage of Lys12ac-H4-labeled spermatids was similar for all groups. Hypac-H4 and Lys12ac-H4 processes were highly correlated in spermatogonia but not in spermatids. Conclusion(s): The reduced percentage of spermatogonia with Hypac-H4 and Lys12ac-H4 in groups B and C may contribute to lower sperm production in mixed atrophy. Spermatids Hypac-H4 impairment in mixed atrophy did not deteriorate further by AZFc region deletion.

Original language	English
Pages (from-to)	1728-1736
Number of pages	9
Journal	Fertility and Sterility
Volume	89
Issue number	6
DOIs	https://doi.org/10.1016/j.fertnstert.2007.05.068
State	Published - Jun 2008
Externally published	Yes

Keywords

AZFc
Azoospermia
histone-H4 acetylation
infertility
spermatogenesis impairments

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10.1016/j.fertnstert.2007.05.068

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title = "Histone H4 acetylation and AZFc involvement in germ cells of specimens of impaired spermatogenesis",

abstract = "Objective: To measure histone-H4 acetylation and involvement of the AZFc region in testicular mixed atrophy. Design: Prospective study. Setting: University-affiliated medical center. Patient(s): Azoospermic men (n = 23) who underwent testicular sperm extraction and preparation for intracytoplasmic sperm injection (ICSI) divided into obstructive azoospermia with complete spermatogenesis (group A), testicular mixed atrophy (group B), and testicular mixed atrophy associated with AZFc deletion (group C). Intervention(s): Testicular biopsy evaluation by Western blotting and quantitative immunohistochemistry of histone-H4 hyperacetylation (Hypac-H4) and lysine-12 acetylation (Lys12ac-H4). Main Outcome Measure(s): Percentage of spermatogonia and spermatids stained by Hypac-H4 and Lys12ac-H4 antibodies in retrieved specimens. Result(s): The percentage of spermatogonia stained for Hypac-H4 and Lys12ac-H4 in groups B and C was statistically significantly reduced. The percentage of elongated spermatids showing positive staining to Hypac-H4 was statistically significantly lower in group B than group A. The percentage of Lys12ac-H4-labeled spermatids was similar for all groups. Hypac-H4 and Lys12ac-H4 processes were highly correlated in spermatogonia but not in spermatids. Conclusion(s): The reduced percentage of spermatogonia with Hypac-H4 and Lys12ac-H4 in groups B and C may contribute to lower sperm production in mixed atrophy. Spermatids Hypac-H4 impairment in mixed atrophy did not deteriorate further by AZFc region deletion.",

keywords = "AZFc, Azoospermia, histone-H4 acetylation, infertility, spermatogenesis impairments",

author = "Kleiman, {Sandra E.} and {Bar-Shira Maymon}, Batia and Ron Hauser and Amnon Botchan and Gedalia Paz and Haim Yavetz and Leah Yogev",

note = "Funding Information: The authors thank Esther Eshkol for editorial assistance, and Esther Shabtai from the Statistical Service, Tel Aviv Sourasky Medical Center, for the expert statistical assistance. The research was carried out under the auspices of the Alan and ADA Selwyn Chair in Clinical Infertility Research and Molecular Medicine (Melbourne, Australia) granted to one of the authors (G. Paz). ",

year = "2008",

month = jun,

doi = "10.1016/j.fertnstert.2007.05.068",

language = "אנגלית",

volume = "89",

pages = "1728--1736",

journal = "Fertility and Sterility",

issn = "0015-0282",

publisher = "Elsevier Inc.",

number = "6",

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TY - JOUR

T1 - Histone H4 acetylation and AZFc involvement in germ cells of specimens of impaired spermatogenesis

AU - Kleiman, Sandra E.

AU - Bar-Shira Maymon, Batia

AU - Hauser, Ron

AU - Botchan, Amnon

AU - Paz, Gedalia

AU - Yavetz, Haim

AU - Yogev, Leah

N1 - Funding Information: The authors thank Esther Eshkol for editorial assistance, and Esther Shabtai from the Statistical Service, Tel Aviv Sourasky Medical Center, for the expert statistical assistance. The research was carried out under the auspices of the Alan and ADA Selwyn Chair in Clinical Infertility Research and Molecular Medicine (Melbourne, Australia) granted to one of the authors (G. Paz).

PY - 2008/6

Y1 - 2008/6

N2 - Objective: To measure histone-H4 acetylation and involvement of the AZFc region in testicular mixed atrophy. Design: Prospective study. Setting: University-affiliated medical center. Patient(s): Azoospermic men (n = 23) who underwent testicular sperm extraction and preparation for intracytoplasmic sperm injection (ICSI) divided into obstructive azoospermia with complete spermatogenesis (group A), testicular mixed atrophy (group B), and testicular mixed atrophy associated with AZFc deletion (group C). Intervention(s): Testicular biopsy evaluation by Western blotting and quantitative immunohistochemistry of histone-H4 hyperacetylation (Hypac-H4) and lysine-12 acetylation (Lys12ac-H4). Main Outcome Measure(s): Percentage of spermatogonia and spermatids stained by Hypac-H4 and Lys12ac-H4 antibodies in retrieved specimens. Result(s): The percentage of spermatogonia stained for Hypac-H4 and Lys12ac-H4 in groups B and C was statistically significantly reduced. The percentage of elongated spermatids showing positive staining to Hypac-H4 was statistically significantly lower in group B than group A. The percentage of Lys12ac-H4-labeled spermatids was similar for all groups. Hypac-H4 and Lys12ac-H4 processes were highly correlated in spermatogonia but not in spermatids. Conclusion(s): The reduced percentage of spermatogonia with Hypac-H4 and Lys12ac-H4 in groups B and C may contribute to lower sperm production in mixed atrophy. Spermatids Hypac-H4 impairment in mixed atrophy did not deteriorate further by AZFc region deletion.

AB - Objective: To measure histone-H4 acetylation and involvement of the AZFc region in testicular mixed atrophy. Design: Prospective study. Setting: University-affiliated medical center. Patient(s): Azoospermic men (n = 23) who underwent testicular sperm extraction and preparation for intracytoplasmic sperm injection (ICSI) divided into obstructive azoospermia with complete spermatogenesis (group A), testicular mixed atrophy (group B), and testicular mixed atrophy associated with AZFc deletion (group C). Intervention(s): Testicular biopsy evaluation by Western blotting and quantitative immunohistochemistry of histone-H4 hyperacetylation (Hypac-H4) and lysine-12 acetylation (Lys12ac-H4). Main Outcome Measure(s): Percentage of spermatogonia and spermatids stained by Hypac-H4 and Lys12ac-H4 antibodies in retrieved specimens. Result(s): The percentage of spermatogonia stained for Hypac-H4 and Lys12ac-H4 in groups B and C was statistically significantly reduced. The percentage of elongated spermatids showing positive staining to Hypac-H4 was statistically significantly lower in group B than group A. The percentage of Lys12ac-H4-labeled spermatids was similar for all groups. Hypac-H4 and Lys12ac-H4 processes were highly correlated in spermatogonia but not in spermatids. Conclusion(s): The reduced percentage of spermatogonia with Hypac-H4 and Lys12ac-H4 in groups B and C may contribute to lower sperm production in mixed atrophy. Spermatids Hypac-H4 impairment in mixed atrophy did not deteriorate further by AZFc region deletion.

KW - AZFc

KW - Azoospermia

KW - histone-H4 acetylation

KW - infertility

KW - spermatogenesis impairments

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DO - 10.1016/j.fertnstert.2007.05.068

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SN - 0015-0282

VL - 89

SP - 1728

EP - 1736

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 6

ER -

Histone H4 acetylation and AZFc involvement in germ cells of specimens of impaired spermatogenesis

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Keywords

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