Histone H4 acetylation and AZFc involvement in germ cells of specimens of impaired spermatogenesis

Sandra E. Kleiman*, Batia Bar-Shira Maymon, Ron Hauser, Amnon Botchan, Gedalia Paz, Haim Yavetz, Leah Yogev

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Objective: To measure histone-H4 acetylation and involvement of the AZFc region in testicular mixed atrophy. Design: Prospective study. Setting: University-affiliated medical center. Patient(s): Azoospermic men (n = 23) who underwent testicular sperm extraction and preparation for intracytoplasmic sperm injection (ICSI) divided into obstructive azoospermia with complete spermatogenesis (group A), testicular mixed atrophy (group B), and testicular mixed atrophy associated with AZFc deletion (group C). Intervention(s): Testicular biopsy evaluation by Western blotting and quantitative immunohistochemistry of histone-H4 hyperacetylation (Hypac-H4) and lysine-12 acetylation (Lys12ac-H4). Main Outcome Measure(s): Percentage of spermatogonia and spermatids stained by Hypac-H4 and Lys12ac-H4 antibodies in retrieved specimens. Result(s): The percentage of spermatogonia stained for Hypac-H4 and Lys12ac-H4 in groups B and C was statistically significantly reduced. The percentage of elongated spermatids showing positive staining to Hypac-H4 was statistically significantly lower in group B than group A. The percentage of Lys12ac-H4-labeled spermatids was similar for all groups. Hypac-H4 and Lys12ac-H4 processes were highly correlated in spermatogonia but not in spermatids. Conclusion(s): The reduced percentage of spermatogonia with Hypac-H4 and Lys12ac-H4 in groups B and C may contribute to lower sperm production in mixed atrophy. Spermatids Hypac-H4 impairment in mixed atrophy did not deteriorate further by AZFc region deletion.

Original languageEnglish
Pages (from-to)1728-1736
Number of pages9
JournalFertility and Sterility
Issue number6
StatePublished - Jun 2008
Externally publishedYes


FundersFunder number
Aeronautical Development Agency


    • AZFc
    • Azoospermia
    • histone-H4 acetylation
    • infertility
    • spermatogenesis impairments


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