TY - JOUR
T1 - Histomorphometric evaluation of natural mineral combined with a synthetic cell-binding peptide (P-15) in critical-size defects in the rat calvaria
AU - Artzi, Zvi
AU - Kozlovsky, Avital
AU - Nemcovsky, Carlos E.
AU - Moses, Ofer
AU - Tal, Haim
AU - Rohrer, Michael D.
AU - Prasad, Hari S.
AU - Weinreb, Miron
PY - 2008
Y1 - 2008
N2 - Purpose: The objective of this study was to histomorphometrically evaluate the synthetic peptide analog P-15 bound to anorganic bovine mineral (Pepgen/P15) in critical-size defects in the rat calvaria. Materials and Methods: A 5-mm-diameter critical-size defect was prepared in 48 rat skulls and divided into 4 equal groups: Pepgen/P15 particles covered by a membrane, Pepgen/P15 particles uncovered, nongrafted membrane-protected sites, and nongrafted uncovered control sites. At 12 weeks, histomorphometric measurements were made of the percentage area of newly formed bone and residual particles, the length of internal and external bone bridging, and linearly, the regenerated marginal and central total tissue augmentation height. Results: Nongrafted, membrane-protected sites gained 60.6% of newly formed bone, followed by 50.6% and 44.2% (P < .05 versus membrane only) at the grafted covered and uncovered sites, respectively. All experimental sites contained significantly (P < .005) more bone than did control sites (19.9%). In both types of grafted sites, the percentage area of Pepgen/P15 particles was similar. Mean internal and external length of bone bridging at nongrafted membrane-protected sites (76.7% and 71.2%, respectively) was significantly greater (P < .005) than that of the grafted covered (43.95% and 51.8%, respectively), grafted uncovered (28.7% and 23.9%, respectively), and control (28% and 25.5%, respectively) groups, except for internal bone bridging in the grafted covered sites. Regenerated marginal and central augmentation heights (0.92 mm and 1.02 mm, respectively) were greatest in the grafted covered group, followed by the non- grafted membrane-protected (0.88 mm and 0.51 mm, respectively), and grafted uncovered (0.89 mm and 0.12 mm, respectively) groups, all of which were significantly greater (P < .001) than the control group (0.63 mm and 0.04 mm, respectively). Conclusion: While anorganic bovine mineral/cell-binding peptide contributes in volume, membrane application significantly increases the amount of bone regeneration. Int j Oral Maxillofac Implant 2008;23:1063-1070.
AB - Purpose: The objective of this study was to histomorphometrically evaluate the synthetic peptide analog P-15 bound to anorganic bovine mineral (Pepgen/P15) in critical-size defects in the rat calvaria. Materials and Methods: A 5-mm-diameter critical-size defect was prepared in 48 rat skulls and divided into 4 equal groups: Pepgen/P15 particles covered by a membrane, Pepgen/P15 particles uncovered, nongrafted membrane-protected sites, and nongrafted uncovered control sites. At 12 weeks, histomorphometric measurements were made of the percentage area of newly formed bone and residual particles, the length of internal and external bone bridging, and linearly, the regenerated marginal and central total tissue augmentation height. Results: Nongrafted, membrane-protected sites gained 60.6% of newly formed bone, followed by 50.6% and 44.2% (P < .05 versus membrane only) at the grafted covered and uncovered sites, respectively. All experimental sites contained significantly (P < .005) more bone than did control sites (19.9%). In both types of grafted sites, the percentage area of Pepgen/P15 particles was similar. Mean internal and external length of bone bridging at nongrafted membrane-protected sites (76.7% and 71.2%, respectively) was significantly greater (P < .005) than that of the grafted covered (43.95% and 51.8%, respectively), grafted uncovered (28.7% and 23.9%, respectively), and control (28% and 25.5%, respectively) groups, except for internal bone bridging in the grafted covered sites. Regenerated marginal and central augmentation heights (0.92 mm and 1.02 mm, respectively) were greatest in the grafted covered group, followed by the non- grafted membrane-protected (0.88 mm and 0.51 mm, respectively), and grafted uncovered (0.89 mm and 0.12 mm, respectively) groups, all of which were significantly greater (P < .001) than the control group (0.63 mm and 0.04 mm, respectively). Conclusion: While anorganic bovine mineral/cell-binding peptide contributes in volume, membrane application significantly increases the amount of bone regeneration. Int j Oral Maxillofac Implant 2008;23:1063-1070.
KW - Bovine bone mineral
KW - Cell-binding peptide
KW - Critical-size defect
KW - Guided bone regeneration
KW - Histomorphometry
KW - PepGen/P-15
UR - http://www.scopus.com/inward/record.url?scp=66149098935&partnerID=8YFLogxK
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AN - SCOPUS:66149098935
SN - 0882-2786
VL - 23
SP - 1063
EP - 1070
JO - International Journal of Oral and Maxillofacial Implants
JF - International Journal of Oral and Maxillofacial Implants
IS - 6
ER -