TY - JOUR
T1 - Histological evaluation of the effects of growth factors in a fibrin network on bone regeneration
AU - Cakir, Sevki
AU - Alper Gultekin, B.
AU - Karabagli, Murat
AU - Yilmaz, Tugba Ekiz
AU - Cakir, Esra
AU - Elif Guzel, E.
AU - Yalcin, Serdar
AU - Mortellaro, Carmen
AU - Mijiritsky, Eitan
N1 - Publisher Copyright:
© 2019 by Mutaz B. Habal, MD.
PY - 2019
Y1 - 2019
N2 - Objective: The aim of this study was to evaluate the effect of mineralized plasmatic matrix (MPM), comprising a combination of synthetic graft and platelet concentrates, on bone regeneration. Methods: Critical size defects of 6-mm diameter were created on the tibias of 6 male sheep, with the animals subsequently assigned into 2 groups. Of the 5 bone defects generated per animal, 4 were randomly filled with MPM, beta-tricalcium phosphate graft (b-TCP), platelet-rich fibrin (PRF) þ b-TCP, and autogenous graft. One defect was left empty as a control group. Animals were killed at 3 weeks (early healing group) and 6 weeks (late healing group). The specimens underwent histologic and histomorphometric analysis to evaluate new bone formation. Results: In both healing periods, new bone formation from autogenous bone was observed significantly more often than from biomaterials or the empty defect. The degree of new bone formation for MPM was significantly higher than that of the control group at all healing periods. In addition, it was significantly higher in both healing periods than that of b-TCP albeit only in the late healing period than that of the PRF þ b-TCP combination. In all biomaterial groups, residual graft ratios decreased from early to late healing periods. Conclusion: The results indicated that MPM, representing growth factors in a fibrin network, increases new bone formation in surgically created defects in sheep tibia as confirmed by histologic assessment.
AB - Objective: The aim of this study was to evaluate the effect of mineralized plasmatic matrix (MPM), comprising a combination of synthetic graft and platelet concentrates, on bone regeneration. Methods: Critical size defects of 6-mm diameter were created on the tibias of 6 male sheep, with the animals subsequently assigned into 2 groups. Of the 5 bone defects generated per animal, 4 were randomly filled with MPM, beta-tricalcium phosphate graft (b-TCP), platelet-rich fibrin (PRF) þ b-TCP, and autogenous graft. One defect was left empty as a control group. Animals were killed at 3 weeks (early healing group) and 6 weeks (late healing group). The specimens underwent histologic and histomorphometric analysis to evaluate new bone formation. Results: In both healing periods, new bone formation from autogenous bone was observed significantly more often than from biomaterials or the empty defect. The degree of new bone formation for MPM was significantly higher than that of the control group at all healing periods. In addition, it was significantly higher in both healing periods than that of b-TCP albeit only in the late healing period than that of the PRF þ b-TCP combination. In all biomaterial groups, residual graft ratios decreased from early to late healing periods. Conclusion: The results indicated that MPM, representing growth factors in a fibrin network, increases new bone formation in surgically created defects in sheep tibia as confirmed by histologic assessment.
KW - Bone augmentation
KW - Bone regeneration
KW - Fibrin network
KW - Growth factors
KW - Platelet concentration
UR - http://www.scopus.com/inward/record.url?scp=85067510527&partnerID=8YFLogxK
U2 - 10.1097/SCS.0000000000005339
DO - 10.1097/SCS.0000000000005339
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C2 - 30839463
AN - SCOPUS:85067510527
SN - 1049-2275
VL - 30
SP - 1078
EP - 1084
JO - Journal of Craniofacial Surgery
JF - Journal of Craniofacial Surgery
IS - 4
ER -