Histochemical demonstration of thiols and disulfides by the fluorescent labeling agent, monobromobimane: An application to the hypothalamo-neurohypophysial system

H. Gainer, N. S. Kosower*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The use of the new fluorescent labeling agent monobrombimane for the histological visualization of thiols, and, after reduction, of disulfides, is described. Fluorescent labeling of the disulfide-rich neurosecretory proteins in the hypothalamo-neurohypophysial system of rat and frog is shown. The reaction of the non-fluorescent reagent with thiols results in highly fluorescent materials which resist fading. The labeling procedure is very simple, rapid, and is carried out in one step. It is non-destructive and can allow for biochemical analyses of the labeled tissue.

Original languageEnglish
Pages (from-to)309-315
Number of pages7
JournalHistochemistry
Volume68
Issue number3
DOIs
StatePublished - Jan 1980

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