Histamine-evoked acetylcholine release in sensitized tracheal preparation

Nir Barak, Rachel Rubinstein, Sasson Cohen

Research output: Contribution to journalArticlepeer-review

Abstract

The contractile response to histamine of tracheal muscle was studied in preparations from BSA-sensitized and non-sensitized guinea-pigs. Sensitization,did not enhance the overall response to histamine. However, this response showed evidence of acetylcholine participation. In sensitized preparations, atropine (0.1 μM) caused a significant depression of the dose response to histamine (n = 11, p = 0.028), especially in tile range 2-8 μM. Physostigmine (0.1 μM) significantly potentiated the effect of histamine (n = 8, p = 0.003), especially at greater than 4 μM histamine. The response to histamine of non-sensitized preparations was not altered by atropine (n = 11) or physostigmine (n = 8). The following agents did not discriminate between sensitized and non-sensitized preparations: Famotidine, an H2 antagonist; dimaprit, an H2 agonist; thioperamide, an H3 antagonist; α-methylhistamine; an H3 agonist; gallamine, an M2 antagonist, suggesting that muscarinic M, receptor dysfunction alone is not sufficient to cause bronchial hyper-responsiveness. The results show that sensitization causes a change ill the components of the contractile response to histamine rather than bronchial hyper-responsiveness to this agent.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalRespiration Physiology
Volume108
Issue number2
DOIs
StatePublished - May 1997

Keywords

  • BSA
  • Guinea pig
  • Histamine
  • Mammals
  • Mediator
  • Sensitization
  • Tracheal muscle
  • Upper airways

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