High voltage pulsed electric field and electroporation technologies for algal biomass processing

Mrinal Kashyap*, Supratim Ghosh, Kiran Bala, Alexander Golberg*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


The production of animal and terrestrial plant proteins has environmental impacts that could limit their sustainable supply. Proteins from microalgae and macroalgae can support the growing animal-free protein demand sustainably, using non-arable land and seawater. However, current technologies and processes developed for terrestrial plant protein processing have multiple limitations when applied to micro and macroalgae, thus, providing an opportunity for emerging technologies. Here we critically review various methods used for micro and macroalgal cell disruption for protein extraction. The pulsed electric field (PEF) algal biomass process is focused on further and highlighted as a green approach for protein extraction from algae. The potential of high-voltage pulsed electric fields and electroporation technologies for algal protein processing are discussed in detail. Current state of the art, challenges, and perspectives are also discussed. The current review will aid in establishing PEF for processing algal biomass to extract valuable compounds including proteins using a non-thermal and chemical-free approach, thus providing an environmentally friendly solution for protein extraction from algae along with highlighting upscaling and processing challenges.

Original languageEnglish
Pages (from-to)273-289
Number of pages17
JournalJournal of Applied Phycology
Issue number1
StatePublished - Feb 2024


FundersFunder number
Good Food Institute
Tel Aviv University
Council for Higher Education
School of Earth and Environmental Sciences, University of St Andrews


    • Algae
    • Electroporation
    • Emerging technologies
    • Extraction
    • Protein
    • Pulsed electric field


    Dive into the research topics of 'High voltage pulsed electric field and electroporation technologies for algal biomass processing'. Together they form a unique fingerprint.

    Cite this