@article{1e191242d7a44f2f9b1490a99290e37f,
title = "High-throughput screening of cellular features using high-resolution light-microscopy; Application for profiling drug effects on cell adhesion",
abstract = "High-resolution light-microscopy and high-throughput screening are two essential methodologies for characterizing cellular phenotypes. Optimally combining these methodologies in cell-based screening to test detailed molecular and cellular responses to multiple perturbations constitutes a major challenge. Here we describe the development and application of a screening microscope platform that automatically acquires and interprets sub-micron resolution images at fast rates. The analysis pipeline is based on the quantification of multiple subcellular features and statistical comparisons of their distributions in treated vs. control cells. Using this platform, we screened 2200 natural extracts for their effects on the fine structure and organization of focal adhesions. This screen identified 15 effective extracts whose fractionation and characterization were further analyzed using the same microscope system. The significance of combining resolution, throughput and multi-parametric analyses for biomedical research and drug discovery is discussed.",
keywords = "Cell-based screening, Focal adhesion, High-resolution light-microscopy, Image analysis, Natural compounds",
author = "Yael Paran and Micha Ilan and Yoel Kashman and Sofee Goldstein and Yuvalal Liron and Benjamin Geiger and Zvi Kam",
note = "Funding Information: We thank Dr. Irena Lavelin for the paxillin-YFP pBabe plasmid and for help in establishing the reporter cell line, and Prof. Edna Schechtman for consulting on statistical methods. The research reported here is supported by grants from the Kahn Fund for Systems Biology at the Weizmann Institute of Science (Z.K.), the NIGMS, National Institutes of Health Cell Migration Consortium Grant U54 GM64346 (B.G.), the Israel Science Foundation (B.G.), Yad Abraham Center for Cancer Diagnosis and Therapy, and the Clore Center for Biological Physics (Z.K.). B.G. holds the E. Neter chair in Cell and Tumor Biology, Z.K. is the Israel Pollak Professor of Biophysics. ",
year = "2007",
month = may,
doi = "10.1016/j.jsb.2006.12.013",
language = "אנגלית",
volume = "158",
pages = "233--243",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "2",
}