High-throughput screening of cellular features using high-resolution light-microscopy; Application for profiling drug effects on cell adhesion

Yael Paran, Micha Ilan, Yoel Kashman, Sofee Goldstein, Yuvalal Liron, Benjamin Geiger, Zvi Kam

Research output: Contribution to journalArticlepeer-review

Abstract

High-resolution light-microscopy and high-throughput screening are two essential methodologies for characterizing cellular phenotypes. Optimally combining these methodologies in cell-based screening to test detailed molecular and cellular responses to multiple perturbations constitutes a major challenge. Here we describe the development and application of a screening microscope platform that automatically acquires and interprets sub-micron resolution images at fast rates. The analysis pipeline is based on the quantification of multiple subcellular features and statistical comparisons of their distributions in treated vs. control cells. Using this platform, we screened 2200 natural extracts for their effects on the fine structure and organization of focal adhesions. This screen identified 15 effective extracts whose fractionation and characterization were further analyzed using the same microscope system. The significance of combining resolution, throughput and multi-parametric analyses for biomedical research and drug discovery is discussed.

Original languageEnglish
Pages (from-to)233-243
Number of pages11
JournalJournal of Structural Biology
Volume158
Issue number2
DOIs
StatePublished - May 2007

Keywords

  • Cell-based screening
  • Focal adhesion
  • High-resolution light-microscopy
  • Image analysis
  • Natural compounds

Fingerprint

Dive into the research topics of 'High-throughput screening of cellular features using high-resolution light-microscopy; Application for profiling drug effects on cell adhesion'. Together they form a unique fingerprint.

Cite this