@article{3ab12c99afa747c89863b7b370c3de84,
title = "High-throughput detection of RNA modifications at single base resolution",
abstract = "RNA is modified by > 170 chemical modifications that affect its structure and function. Accordingly, RNA modifications have been implicated in regulation of gene expression and cellular outcomes in a variety of species spanning the phylogenetic tree. The study of RNA modifications is accelerated by generation of high-throughput methods for detecting RNA modifications at single base resolution. Here, we review recent advancement in next generation sequencing based approaches for detection of 14 distinct RNA modifications present in rRNA, tRNA and mRNA. We further outline the molecular and computational principles underlying currently available methods.",
keywords = "RNA modifications, epitranscriptome, mass-spectrometry, nanopore, next-generation sequencing",
author = "Keren Ron and Joshua Kahn and Nofar Malka-Tunitsky and Aldema Sas-Chen",
note = "Publisher Copyright: {\textcopyright} 2024 The Author(s). FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.",
year = "2025",
month = jan,
doi = "10.1002/1873-3468.15052",
language = "אנגלית",
volume = "599",
pages = "19--32",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "1",
}