TY - JOUR
T1 - High-performance liquid chromatographic detection of pitfalls in porphobilinogen deaminase determination
AU - Schoenfeld, Nili
AU - Mamet, Rivka
PY - 1991/9/18
Y1 - 1991/9/18
N2 - Potential pitfalls in the determination of porphobilinogen deaminase activity, as well as ways of eliminating these sources of error and determining the activity accurately, are discussed. In addition to measurement of the accurate activity, the described method (a combination of incubation of homogenate with porphobilinogen and high-performance liquid chromatographic separation) can also be used to detect enzymic defects in the haem biosynthetic pathway, according to the pattern of accumulation of the various porphyrins.
AB - Potential pitfalls in the determination of porphobilinogen deaminase activity, as well as ways of eliminating these sources of error and determining the activity accurately, are discussed. In addition to measurement of the accurate activity, the described method (a combination of incubation of homogenate with porphobilinogen and high-performance liquid chromatographic separation) can also be used to detect enzymic defects in the haem biosynthetic pathway, according to the pattern of accumulation of the various porphyrins.
UR - http://www.scopus.com/inward/record.url?scp=0025787128&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(91)80200-V
DO - 10.1016/0378-4347(91)80200-V
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AN - SCOPUS:0025787128
SN - 0378-4347
VL - 570
SP - 51
EP - 64
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -