High la (HLA‐DR) and low CD11b (Mo1) expression may predict early conversion to leukemia in myelodysplastic syndromes

Moshe Mittelman, Donald S. Karcher, Lisa A. Kammerman, Lawrence S. Lessin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The FAB classification of myelodysplastic syndromes (MDS) has been useful in predicting prognosis; however, additional methods are required to detect patients at high risk for early conversion to acute nonlymphoblastic leukemia (ANLL). Using a panel of monoclonal antibodies to myelomonocytic surface antigens (MMSA) and flow cytometry, we studied bone marrow cells from 26 patients with MDS of all five FAB subtypes. The MMSA studied included la (HLA‐DR), CD11b (Mo1), CD14 (Mo2, My4), CD13 (My7), and CD33 (My9). Marrows were considered 'positive' for a given MMSA if the percentage of reactive cells exceeded the upper limit of the normal range. Twenty‐four of twenty‐six patients (92.3%) were CD13 (My7)+, suggesting that CD13 may serve as a diagnostic marker for MDS. Ten of twelve patients who developed ANLL during a median follow‐up of 44 weeks were la(HLA‐DR)+. The Kaplan‐Meier estimated median time to leukemia (TTL) was 16 weeks for la+ patients and 88 weeks for la– patients (P = 0.004). All six patients who developed ANLL before 16 weeks from diagnosis were la+, while none of the la– patients converted to ANLL before 24 weeks. Nine of thirteen patients with low CD11b (Mo1) expression (<53% reactive cells) developed ANLL, compared with only two of 11 patients with high CD11b expression (>53% reactive cells). Kaplan‐Meier estimated TTL was 29 weeks for patients with low CD11b, compared to 160 weeks for patients with high CD11b (P < 0.05). Patients who met both criteria, la+ and low CD11b, represented the poorest prognostic subgroup, with median TTL of 13 weeks compared with 88 weeks for the others (P = 0.017), la and CD11b patterns were not specific for MDS subtype, and their expression did not correlate with blast count. These data suggest that MDS patients whose bone marrow cells demonstrate high la (HLA‐DR) and low CD11b (Mo1) expression represent a poor prognostic subgroup with short TTL. These patients may be candidates for early aggressive or investigational treatment.

Original languageEnglish
Pages (from-to)165-171
Number of pages7
JournalAmerican Journal of Hematology
Volume43
Issue number3
DOIs
StatePublished - Jul 1993
Externally publishedYes

Keywords

  • acute nonlymphoblastic leukemia
  • immunochemistry
  • monoclonal antibodies
  • myelomonocytic differentiation antigens

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