@article{e5822ec1e8974f1bb9d2b5132a9929d1,
title = "High efficiency of a sequential recombinase-mediated cassette exchange reaction in escherichia coli",
abstract = "A comparison between the efficiency of recombinase-mediated cassette exchange (RMCE) reactions catalyzed in Escherichia coli by the site-specific recombinases Flp of yeast and Int of coliphage HK022 has revealed that an Flp-catalyzed RMCE reaction is more efficient than an Int-HK022 catalyzed reaction. In contrast, an RMCE reaction with 1 pair of frt sites and 1 pair of att sites catalyzed in the presence of both recombinases is very inefficient. However, the same reaction catalyzed by each recombinase individually supplied in a sequential order is very efficient, regardless of the order. Atomic force microscopy images of Flp with its DNA substrates show that only 1 pair of recombination sites forms a synaptic complex with the recombinase. The results suggest that the RMCE reaction is sequential.",
keywords = "Atomic force microscopy, Coliphage HK022, Flp recombinase, Integrase, Recombinase-mediated cassette exchange, Site-specific recombination",
author = "Natalia Malchin and Tatiana Molotsky and Ilya Borovok and Yuri Voziyanov and Kotlyar, {Alexander B.} and Ezra Yagil and Mikhail Kolot",
year = "2010",
month = dec,
doi = "10.1159/000321497",
language = "אנגלית",
volume = "19",
pages = "117--122",
journal = "Journal of Molecular Microbiology and Biotechnology",
issn = "1464-1801",
publisher = "S. Karger AG",
number = "3",
}