High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva

Zijie Zhang; Richa Pandey; Jiuxing Li; Jimmy Gu; Dawn White; Hannah D. Stacey; Jann C. Ang; Catherine Jean Steinberg; Alfredo Capretta; Carlos D.M. Filipe; Karen Mossman; Cynthia Balion; Matthew S. Miller; Bruno J. Salena; Deborah Yamamura; Leyla Soleymani; John D. Brennan; Yingfu Li

doi:10.1002/anie.202110819

High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva

Zijie Zhang, Richa Pandey, Jiuxing Li, Jimmy Gu, Dawn White, Hannah D. Stacey, Jann C. Ang, Catherine Jean Steinberg, Alfredo Capretta, Carlos D.M. Filipe, Karen Mossman, Cynthia Balion, Matthew S. Miller, Bruno J. Salena, Deborah Yamamura, Leyla Soleymani^*, John D. Brennan^*, Yingfu Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

We report a simple and rapid saliva-based SARS-CoV-2 antigen test that utilizes a newly developed dimeric DNA aptamer, denoted as DSA1N5, that specifically recognizes the spike proteins of the wildtype virus and its Alpha and Delta variants with dissociation constants of 120, 290 and 480 pM, respectively, and binds pseudotyped lentiviruses expressing the wildtype and alpha trimeric spike proteins with affinity constants of 2.1 pM and 2.3 pM, respectively. To develop a highly sensitive test, DSA1N5 was immobilized onto gold electrodes to produce an electrochemical impedance sensor, which was capable of detecting 1000 viral particles per mL in 1:1 diluted saliva in under 10 min without any further sample processing. Evaluation of 36 positive and 37 negative patient saliva samples produced a clinical sensitivity of 80.5 % and specificity of 100 % and the sensor could detect the wildtype virus as well as the Alpha and Delta variants in the patient samples, which is the first reported rapid test that can detect any emerging variant of SARS-CoV-2.

Original language	English
Pages (from-to)	24266-24274
Number of pages	9
Journal	Angewandte Chemie - International Edition
Volume	60
Issue number	45
DOIs	https://doi.org/10.1002/anie.202110819
State	Published - 2 Nov 2021
Externally published	Yes

Keywords

COVID-19
aptamers
electrochemical biosensors
rapid tests
saliva

Access to Document

10.1002/anie.202110819

Cite this

Zhang, Z., Pandey, R., Li, J., Gu, J., White, D., Stacey, H. D., Ang, J. C., Steinberg, C. J., Capretta, A., Filipe, C. D. M., Mossman, K., Balion, C., Miller, M. S., Salena, B. J., Yamamura, D., Soleymani, L., Brennan, J. D., & Li, Y. (2021). High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva. Angewandte Chemie - International Edition, 60(45), 24266-24274. https://doi.org/10.1002/anie.202110819

@article{768bf852fb9349afb366991835f58093,

title = "High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva",

abstract = "We report a simple and rapid saliva-based SARS-CoV-2 antigen test that utilizes a newly developed dimeric DNA aptamer, denoted as DSA1N5, that specifically recognizes the spike proteins of the wildtype virus and its Alpha and Delta variants with dissociation constants of 120, 290 and 480 pM, respectively, and binds pseudotyped lentiviruses expressing the wildtype and alpha trimeric spike proteins with affinity constants of 2.1 pM and 2.3 pM, respectively. To develop a highly sensitive test, DSA1N5 was immobilized onto gold electrodes to produce an electrochemical impedance sensor, which was capable of detecting 1000 viral particles per mL in 1:1 diluted saliva in under 10 min without any further sample processing. Evaluation of 36 positive and 37 negative patient saliva samples produced a clinical sensitivity of 80.5 % and specificity of 100 % and the sensor could detect the wildtype virus as well as the Alpha and Delta variants in the patient samples, which is the first reported rapid test that can detect any emerging variant of SARS-CoV-2.",

keywords = "COVID-19, aptamers, electrochemical biosensors, rapid tests, saliva",

author = "Zijie Zhang and Richa Pandey and Jiuxing Li and Jimmy Gu and Dawn White and Stacey, {Hannah D.} and Ang, {Jann C.} and Steinberg, {Catherine Jean} and Alfredo Capretta and Filipe, {Carlos D.M.} and Karen Mossman and Cynthia Balion and Miller, {Matthew S.} and Salena, {Bruno J.} and Deborah Yamamura and Leyla Soleymani and Brennan, {John D.} and Yingfu Li",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH",

year = "2021",

month = nov,

day = "2",

doi = "10.1002/anie.202110819",

language = "אנגלית",

volume = "60",

pages = "24266--24274",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "45",

}

Zhang, Z, Pandey, R, Li, J, Gu, J, White, D, Stacey, HD, Ang, JC, Steinberg, CJ, Capretta, A, Filipe, CDM, Mossman, K, Balion, C, Miller, MS, Salena, BJ, Yamamura, D, Soleymani, L, Brennan, JD & Li, Y 2021, 'High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva', Angewandte Chemie - International Edition, vol. 60, no. 45, pp. 24266-24274. https://doi.org/10.1002/anie.202110819

TY - JOUR

T1 - High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva

AU - Zhang, Zijie

AU - Pandey, Richa

AU - Li, Jiuxing

AU - Gu, Jimmy

AU - White, Dawn

AU - Stacey, Hannah D.

AU - Ang, Jann C.

AU - Steinberg, Catherine Jean

AU - Capretta, Alfredo

AU - Filipe, Carlos D.M.

AU - Mossman, Karen

AU - Balion, Cynthia

AU - Miller, Matthew S.

AU - Salena, Bruno J.

AU - Yamamura, Deborah

AU - Soleymani, Leyla

AU - Brennan, John D.

AU - Li, Yingfu

PY - 2021/11/2

Y1 - 2021/11/2

N2 - We report a simple and rapid saliva-based SARS-CoV-2 antigen test that utilizes a newly developed dimeric DNA aptamer, denoted as DSA1N5, that specifically recognizes the spike proteins of the wildtype virus and its Alpha and Delta variants with dissociation constants of 120, 290 and 480 pM, respectively, and binds pseudotyped lentiviruses expressing the wildtype and alpha trimeric spike proteins with affinity constants of 2.1 pM and 2.3 pM, respectively. To develop a highly sensitive test, DSA1N5 was immobilized onto gold electrodes to produce an electrochemical impedance sensor, which was capable of detecting 1000 viral particles per mL in 1:1 diluted saliva in under 10 min without any further sample processing. Evaluation of 36 positive and 37 negative patient saliva samples produced a clinical sensitivity of 80.5 % and specificity of 100 % and the sensor could detect the wildtype virus as well as the Alpha and Delta variants in the patient samples, which is the first reported rapid test that can detect any emerging variant of SARS-CoV-2.

AB - We report a simple and rapid saliva-based SARS-CoV-2 antigen test that utilizes a newly developed dimeric DNA aptamer, denoted as DSA1N5, that specifically recognizes the spike proteins of the wildtype virus and its Alpha and Delta variants with dissociation constants of 120, 290 and 480 pM, respectively, and binds pseudotyped lentiviruses expressing the wildtype and alpha trimeric spike proteins with affinity constants of 2.1 pM and 2.3 pM, respectively. To develop a highly sensitive test, DSA1N5 was immobilized onto gold electrodes to produce an electrochemical impedance sensor, which was capable of detecting 1000 viral particles per mL in 1:1 diluted saliva in under 10 min without any further sample processing. Evaluation of 36 positive and 37 negative patient saliva samples produced a clinical sensitivity of 80.5 % and specificity of 100 % and the sensor could detect the wildtype virus as well as the Alpha and Delta variants in the patient samples, which is the first reported rapid test that can detect any emerging variant of SARS-CoV-2.

KW - COVID-19

KW - aptamers

KW - electrochemical biosensors

KW - rapid tests

KW - saliva

UR - http://www.scopus.com/inward/record.url?scp=85116475783&partnerID=8YFLogxK

U2 - 10.1002/anie.202110819

DO - 10.1002/anie.202110819

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 34464491

AN - SCOPUS:85116475783

SN - 1433-7851

VL - 60

SP - 24266

EP - 24274

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 45

ER -

High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this