Hemopoietic activity of stromal CEU. Lines with osteogenic potential isolated from developing ossicles

I. Bleiberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The study was aimed to determine the hemopoietic and osteogenic potential of stromal cell lines. Sixty semiclonal cell tines have been isolated from different stages of ossicle development after subcutaneous implantation of deraraeralized bone matrix (DBM) into rats. Days 3.7 and 14 post implantation were selected for isolation of stromal cells at stages of major mesenchymal cell accumulation, chondrogenesis or osteogeoesis respectively, Cell lines have been examined for their capacity to develop into bone upon subcapsular implantation into iaologous rat kidney, as well as for their osteogenic and hemopoietic potential in vitro. Two of the cell lines developed into bone in the subcapsular site. Implantation of cell lines in the presence of DBM did not increase bone formation. Most cell lines whether of day 3, 7 or 14 origin were found to be alkaline phosphatase positive, and this was stimulated by 1,25 (OH)2 vit. Dj and/or ascorbic acid in some of the lines. Elevated levels of c-AMP were detected in some of these cultures after exposure to Parathyroid hormone. All of the cell lines secreted hemopoietic growth factors) (HGF) into the medium which stimulated development of granulocytic and macrophagic colonies of bone marrow origin in semisolid medium, LPS. vit. D, or IL-1 a stimulated HGF activity. Some of the cell lines have been found to stimulate the survival of bemopoiettc precursors of bone marrow origin in cocultares for up to 96 hours of incubation. This survival was decreased when an agar layer was placed between the cell lines and the bone marrow cells. In conclusion : stromal cell lines of meaenchymal origin isolated from developing ossicles have osteogenic potential and the capacity to stimulate hemopoiesis.

Original languageEnglish
Pages (from-to)1096
Number of pages1
JournalExperimental Hematology
Issue number9
StatePublished - 1996


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