Hemizona assay for evaluating fertilizing capacity of human spermatozoa

R. Gamzu*, L. Yogev, H. Yavetz, Z. T. Homonnai, G. Paz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The hemizona assay (HZA), which tests the binding capacity of human spermatozoa to the zona pellucida, is described. This assay has very high sensitivity (86-100%) in predicting fertilizing capacity in the in vitro fertilization (IVF) system. It is recommended that it be carried out on semen samples before entering the IVF program. In addition, it can also help evaluate unexplained infertility and assess the quality of ova. The necessity for a control fertilized sample in each HZA might complicate the assay. To simplify the test, the use of sperm from the sperm bank was investigated. It was shown that fresh and frozen-thawed sperm have the same capacity to bind to the hemizonae. Thus, frozen donor semen preserved in the sperm bank can be used for controlling the HZA.

Original languageEnglish
Pages (from-to)604-606, 668
Issue number10
StatePublished - 16 May 1993
Externally publishedYes


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