Helium-neon laser irradiation attenuates anoxia-induced degeneration of rabbit retinal ganglion cells

Arieh M.D. Solomon*, Avi M.D. Amir, Vered Lavie

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

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The effect of low-energy laser irradiation, known to delay axonal degeneration in mechanically traumatized nerves, was investigated in rabbit retinal ganglion cells damaged by temporary anoxia. Complete retinal vascular occlusion was induced in 39 rabbits by application of pressure to the cornea, with continuous monitoring under an operating microscope. The duration of occlusion was 15, 30 or 60 minutes. Starting immediately after the cessation of vascular occlusion, half of the rabbits in each group received transcorneal irradiation with a 35-mW helium-neon laser for 5 minutes daily on 10 consecutive days. The nonirradiated rabbits served as controls. Retinal ganglion cell viability was demonstrated by retrograde labeling of their axons with horseradish peroxidase, introduced subdurally into the optic nerve at a distance of 2 mm distal to the globe, 48 hours prior to sacrifice. For labeling intensity controls we used normal, not occluded, animals labeled with horseradish peroxidase by the same method. The animals were sacrificed 2, 4 or 8 weeks after occlusion. Labeling of retinal ganglion cells and their axons was observed in 100% of the normal control animals and in 85% of the irradiated rabbits. The results suggest that low-energy helium-neon laser irradiation attenuates the damage inflicted on the retinal ganglion cells as a result of anoxia.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Society of Photo-Optical Instrumentation Engineers
Number of pages7
ISBN (Print)0819411108
StatePublished - 1993
EventLow-Energy Laser Effects on Biological Systems - Los Angeles, CA, USA
Duration: 19 Jan 199320 Jan 1993

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


ConferenceLow-Energy Laser Effects on Biological Systems
CityLos Angeles, CA, USA


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