TY - JOUR
T1 - Heat-Stress Preconditioning Attenuates Behavioral Responses to Psychological Stress
T2 - The Role of HSP-70 in Modulating Stress Responses
AU - Belity, Tal
AU - Horowitz, Michal
AU - Hoffman, Jay R.
AU - Epstein, Yoram
AU - Bruchim, Yaron
AU - Todder, Doron
AU - Cohen, Hagit
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/4/1
Y1 - 2022/4/1
N2 - Exposure to high ambient temperature is a stressor that influences both biological and behavioral functions and has been previously shown to have an extensive impact on brain structure and function. Physiological, cellular and behavioral responses to heat-stress (HS) (40–41 °C, 2 h) were evaluated in adult male Sprague-Dawley rats. The effect of HS exposure before predator-scent stress (PSS) exposure (i.e., HS preconditioning) was examined. Finally, a possible mechanism of HS-preconditioning to PSS was investigated. Immunohistochemical analyses of chosen cellular markers were performed in the hippocampus and in the hypothalamic paraventricular nucleus (PVN). Plasma corticosterone levels were evaluated, and the behavioral assessment included the elevated plus-maze (EPM) and the acoustic startle response (ASR) paradigms. Endogenous levels of heat shock protein (HSP)-70 were manipulated using an amino acid (L-glutamine) and a pharmacological agent (Doxazosin). A single exposure to an acute HS resulted in decreased body mass (BM), increased body temperature and increased corticosterone levels. Additionally, extensive cellular, but not behavioral changes were noted. HS-preconditioning provided behavioral resiliency to anx-iety-like behavior associated with PSS, possibly through the induction of HSP-70. Targeting of HSP70 is an attractive strategy for stress-related psychopathology treatment.
AB - Exposure to high ambient temperature is a stressor that influences both biological and behavioral functions and has been previously shown to have an extensive impact on brain structure and function. Physiological, cellular and behavioral responses to heat-stress (HS) (40–41 °C, 2 h) were evaluated in adult male Sprague-Dawley rats. The effect of HS exposure before predator-scent stress (PSS) exposure (i.e., HS preconditioning) was examined. Finally, a possible mechanism of HS-preconditioning to PSS was investigated. Immunohistochemical analyses of chosen cellular markers were performed in the hippocampus and in the hypothalamic paraventricular nucleus (PVN). Plasma corticosterone levels were evaluated, and the behavioral assessment included the elevated plus-maze (EPM) and the acoustic startle response (ASR) paradigms. Endogenous levels of heat shock protein (HSP)-70 were manipulated using an amino acid (L-glutamine) and a pharmacological agent (Doxazosin). A single exposure to an acute HS resulted in decreased body mass (BM), increased body temperature and increased corticosterone levels. Additionally, extensive cellular, but not behavioral changes were noted. HS-preconditioning provided behavioral resiliency to anx-iety-like behavior associated with PSS, possibly through the induction of HSP-70. Targeting of HSP70 is an attractive strategy for stress-related psychopathology treatment.
KW - anxiety
KW - heat shock protein-70
KW - heat-stress
KW - hyperthermia
KW - hypothalamus-pituitary-ad-renal axis
KW - preconditioning
UR - http://www.scopus.com/inward/record.url?scp=85127750465&partnerID=8YFLogxK
U2 - 10.3390/ijms23084129
DO - 10.3390/ijms23084129
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C2 - 35456946
AN - SCOPUS:85127750465
SN - 1661-6596
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 8
M1 - 4129
ER -