Healing capacity of bone surrounding biofilm-infected and non-infected gutta-percha: A study of rat calvaria

Daniel Moreinos, Ronald Wigler, Yuval Geffen, Sharon Akrish, Shaul Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


This paper aims to evaluate the healing capacity of bony lesions around biofilm-infected and non-infected gutta-percha (GP) points. Bony defects were created in the calvaria of 28 Wistar rats. The rats were divided into three groups: Group 1—Implantation of infected GP particles in the bony defect; Group 2—Positive control implantation of non-infected GP particles in the bony defect; and Group 3—Negative control, in which no GP particles were implanted. The biofilm consisted of three strains of bacteria: Enterococcus faecalis, Streptococcus sanguis, and Porphyromonas gingivalis. The animals were sacrificed 60 days postoperation, and histological assessments were performed. In Group 1, the biofilm-infected group, we observed a mild foreign body reaction with a few inflammatory cells adjacent to the capsule and a newly woven bone matrix surrounded by osteoblasts and mature bone. In Group 2, the non-infected GP particles group, minimal inflammatory cell reactions were observed in the adjacent tissue, and a newly woven bone matrix was surrounded by osteoblasts. This study shows that bone healing is possible around both sterile and infected GP points. This contradicts the claim that some root canal treatments fail because of non-microbial factors, including extruded root canal filling materials, which may cause foreign body reactions. The healing observed suggests that overextension should not be considered an indication for endodontic surgery.

Original languageEnglish
Article number6710
JournalApplied Sciences (Switzerland)
Issue number15
StatePublished - 1 Aug 2021


FundersFunder number
MIS Company Implants Technologies Ltd.


    • Biofilm
    • Bone healing capacity
    • Infected gutta-percha
    • Rat calvarium


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