TY - JOUR
T1 - Harmonization of the Bayer ADVIA Centaur and Abbott AxSYM automated B-type natriuretic peptide assay in patients on hemodialysis
AU - Barak, Mira
AU - Weinberger, Ronit
AU - Marcusohn, Jerom
AU - Froom, Paul
PY - 2005/5
Y1 - 2005/5
N2 - There are two fully automated high-throughput clinical instruments for brain natriuretic peptide (BNP) assays, the Bayer ADVIA Centaur assay, and the Abbott AxSYM assay. Although both recommend a cut-off value of 100 pg/mL, we are unaware of previous studies that have compared the unadjusted results of the two methods, required for proper evaluation of patients undergoing this test on different platforms. From 43 hemodialysis patients, 80 paired samples were collected by venipuncture into plastic evacuated tubes containing EDTA. The Bayer assay yielded lower values than the Abbott assay, with linear regression of 0.53 × Abbott assay (95% confidence interval, 0.50-0.56) being forced through 0, demonstrating an r2-value of 0.954. Regression for the Abbott assay was 1.79 × Bayer assay (95% CI, 1.69-1.89). The cut-off values for abnormal BNP results analyzed on the Abbott system are not identical to those on the Bayer system, and this needs to be taken into account when comparing studies on the clinical utility of these systems.
AB - There are two fully automated high-throughput clinical instruments for brain natriuretic peptide (BNP) assays, the Bayer ADVIA Centaur assay, and the Abbott AxSYM assay. Although both recommend a cut-off value of 100 pg/mL, we are unaware of previous studies that have compared the unadjusted results of the two methods, required for proper evaluation of patients undergoing this test on different platforms. From 43 hemodialysis patients, 80 paired samples were collected by venipuncture into plastic evacuated tubes containing EDTA. The Bayer assay yielded lower values than the Abbott assay, with linear regression of 0.53 × Abbott assay (95% confidence interval, 0.50-0.56) being forced through 0, demonstrating an r2-value of 0.954. Regression for the Abbott assay was 1.79 × Bayer assay (95% CI, 1.69-1.89). The cut-off values for abnormal BNP results analyzed on the Abbott system are not identical to those on the Bayer system, and this needs to be taken into account when comparing studies on the clinical utility of these systems.
KW - Abbott
KW - Automated
KW - Bayer
KW - Brain natriuretic peptide
KW - Harmonization
KW - Reference range
UR - http://www.scopus.com/inward/record.url?scp=23044459546&partnerID=8YFLogxK
U2 - 10.1515/CCLM.2005.096
DO - 10.1515/CCLM.2005.096
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AN - SCOPUS:23044459546
SN - 1434-6621
VL - 43
SP - 554
EP - 556
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
IS - 5
ER -