Haplotyping a non-meiotic diploid fungal pathogen using induced aneuploidies and SNP/CGH microarray analysis

Judith Berman, Anja Forche*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The generation of haplotype information has recently become very attractive due to its utility for identifying mutations associated with human disease and for the development of personalized medicine. Haplotype information also is crucial for studying recombination mechanisms and genetic diversity, and for analyzing allele-specific gene expression. Classic haplotyping methods require the analysis of hundreds of meiotic progeny. To facilitate haplotyping in the non-meiotic human fungal pathogen Candida albicans, we exploited trisomic heterozygous chromosomes generated via the UAU1 selection strategy. Using this system, we obtained phasing information from allelic biases, detected by SNP/CGH microarray analysis. This strategy has the potential to be applicable to other diploid, asexual Candida species that are important causes of human disease.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages131-146
Number of pages16
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1551
ISSN (Print)1064-3745

Keywords

  • Aneuploidy
  • Candida albicans
  • Haplotyping
  • Heterozygosity
  • Non-meiotic organism
  • Phased genome
  • SNP/CGH microarrays
  • UAU1 cassette
  • Ymap

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