TY - JOUR
T1 - Halophyte biorefinery for polyhydroxyalkanoates production from Ulva sp. Hydrolysate with Haloferax mediterranei in pneumatically agitated bioreactors and ultrasound harvesting
AU - Ghosh, Supratim
AU - Coons, Jim
AU - Yeager, Chris
AU - Halley, Peter
AU - Chemodanov, Alexander
AU - Belgorodsky, Bogdan
AU - Gozin, Michael
AU - Chen, Guo Qiang
AU - Golberg, Alexander
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2022/1
Y1 - 2022/1
N2 - The present study tested the outdoor cultivation of Haloferax mediterranei for PHA production from green macroalgae Ulva sp. in pneumatically agitated bioreactors and applied ultrasonic separation for enhanced settling of archaeal cells. Scaled-up cultivation (40 L) yielded maximum biomass productivity of 50.1 ± 0.11 mg·L-1·h−1 with a PHA productivity of 27 ± 0.01 mg·L-1·h−1 and conversion yield of 0.107 g PHA per gram UlvaDW. The maximum mass fraction of PHA achieved in biomass was calculated to be 56% w/w. Ultrasonic harvesting of Hfx. mediterranei cells approached 30% removal at energy inputs around 7.8 kWh·m−3, and indicated no significant aggregation enhancement by Ca2+ addition. Molecular weight analysis showed an increase in Polydispersity Index (PDI) when the corresponding air velocities were increased suggesting that the polymer was more homogeneous at lower mixing velocities. The current study demonstrated scalable processes for PHA production using Ulva sp. feedstock providing new technologies for halophilic biorefinery.
AB - The present study tested the outdoor cultivation of Haloferax mediterranei for PHA production from green macroalgae Ulva sp. in pneumatically agitated bioreactors and applied ultrasonic separation for enhanced settling of archaeal cells. Scaled-up cultivation (40 L) yielded maximum biomass productivity of 50.1 ± 0.11 mg·L-1·h−1 with a PHA productivity of 27 ± 0.01 mg·L-1·h−1 and conversion yield of 0.107 g PHA per gram UlvaDW. The maximum mass fraction of PHA achieved in biomass was calculated to be 56% w/w. Ultrasonic harvesting of Hfx. mediterranei cells approached 30% removal at energy inputs around 7.8 kWh·m−3, and indicated no significant aggregation enhancement by Ca2+ addition. Molecular weight analysis showed an increase in Polydispersity Index (PDI) when the corresponding air velocities were increased suggesting that the polymer was more homogeneous at lower mixing velocities. The current study demonstrated scalable processes for PHA production using Ulva sp. feedstock providing new technologies for halophilic biorefinery.
KW - Haloferax mediterranei, pneumatically agitated bioreactors
KW - Halophyte biorefinery
KW - Polyhydroxyalkanoates
KW - Ultrasonic separation
UR - http://www.scopus.com/inward/record.url?scp=85120902105&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2021.125964
DO - 10.1016/j.biortech.2021.125964
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C2 - 34728090
AN - SCOPUS:85120902105
SN - 0960-8524
VL - 344
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 125964
ER -