Halofuginone: A potent inhibitor of critical steps in angiogenesis progression

M. Elkin, H. Q. Miao, A. Nagler, E. Aingorn, R. Reich, I. Hemo, H. L. Dou, M. Pines, I. Vlodavsky

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously demonstrated that halofuginone, a low molecular weight quinazolinone alkaloid, is a potent inhibitor of collagen cd (I) and matrix metalloproteinase 2 (MMP-2) gene expression. Halofuginone also effectively suppresses tumor progression and metastasis in mice. These results together with the well-documented role of extracellular matrix (ECM) components and matrix degrading enzymes in formation of new blood vessels led us to investigate the effect of halofuginone on the anglogenic process. In a variety of experimental system, representing sequential events in the angiogenic cascade, halofuginone treatment resulted in profound inhibitory effect. Among these are the abrogation of endothelial cell MMP-2 expression and basement membrane invasion, capillary tube formation, and vascular sprouting, as well as deposition of subendothelial ECM. The most conclusive anti-angiogenic activity of halofuginone was demonstrated in vivo (mouse corneal micropocket assay) by showing a marked inhibition of basic fibroblast growth factor (bFGF) -induced neovascularization in response to systemic administration of halofuginone, either i.p. or in the diet. The ability of halofuginone to interfere with key events in neovascularization, together with its oral bioavailability and safe use as an anti-parasitic agent, make it a promising drug for further evaluation in the treatment of a wide range of diseases associated with pathological angiogenesis.

Original languageEnglish
Pages (from-to)2477-2485
Number of pages9
JournalFASEB Journal
Volume14
Issue number15
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Extracellular matrix
  • Halofuginone
  • Matrix metalloproteinase-2
  • Neovascularization
  • Type I collagen

Fingerprint

Dive into the research topics of 'Halofuginone: A potent inhibitor of critical steps in angiogenesis progression'. Together they form a unique fingerprint.

Cite this