TY - JOUR
T1 - Guanosine potentiates the antiproliferative effect of cytosine-βd-arabinofuranoside in melanoma cell lines
AU - Sidi, Y.
AU - Panet, C.
AU - Cyjon, A.
AU - Fenig, E.
AU - Beery, E.
AU - Nordenberg, J.
N1 - Funding Information:
Dr. Zeev Keinan is acknowledged for his help in the TLC experiments. This study was supported by the Schreiber and the Ashkenazi Cancer Research Institute Funds, Tel-Aviv University.
PY - 1993
Y1 - 1993
N2 - Guanosine is shown to potentiate markedly the antiproliferative effect of cytosine-βD-arabinoside (ara-C) on B16 F10 mouse and SKMEL-28 human melanoma cell lines. Several metabolic consequences of the synergistic interaction between ara-C and guanosine on cell growth were determined in BI6 F10 mouse melanoma cells. Treatment of the cells with guanosine for 24hr resulted in an increase in the percentage of cells in the S phase of the cell cycle, a threefold increase in intracellular GTP concentration, and an increase in the incorporation of ara-C into acid-insoluble material and phosphorylated metabolites. These findings suggest that guanosine potentiates the growth-inhibitory effect of ara-C in B16 F10 melanoma cells by increasing the intracellular concentration of its active metabolites.
AB - Guanosine is shown to potentiate markedly the antiproliferative effect of cytosine-βD-arabinoside (ara-C) on B16 F10 mouse and SKMEL-28 human melanoma cell lines. Several metabolic consequences of the synergistic interaction between ara-C and guanosine on cell growth were determined in BI6 F10 mouse melanoma cells. Treatment of the cells with guanosine for 24hr resulted in an increase in the percentage of cells in the S phase of the cell cycle, a threefold increase in intracellular GTP concentration, and an increase in the incorporation of ara-C into acid-insoluble material and phosphorylated metabolites. These findings suggest that guanosine potentiates the growth-inhibitory effect of ara-C in B16 F10 melanoma cells by increasing the intracellular concentration of its active metabolites.
UR - http://www.scopus.com/inward/record.url?scp=0027317749&partnerID=8YFLogxK
U2 - 10.3109/07357909309011670
DO - 10.3109/07357909309011670
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C2 - 8402221
AN - SCOPUS:0027317749
SN - 0735-7907
VL - 11
SP - 523
EP - 529
JO - Cancer Investigation
JF - Cancer Investigation
IS - 5
ER -
