Guanosine potentiates the antiproliferative effect of cytosine-βd-arabinofuranoside in melanoma cell lines

Y. Sidi, C. Panet, A. Cyjon, E. Fenig, E. Beery, J. Nordenberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Guanosine is shown to potentiate markedly the antiproliferative effect of cytosine-βD-arabinoside (ara-C) on B16 F10 mouse and SKMEL-28 human melanoma cell lines. Several metabolic consequences of the synergistic interaction between ara-C and guanosine on cell growth were determined in BI6 F10 mouse melanoma cells. Treatment of the cells with guanosine for 24hr resulted in an increase in the percentage of cells in the S phase of the cell cycle, a threefold increase in intracellular GTP concentration, and an increase in the incorporation of ara-C into acid-insoluble material and phosphorylated metabolites. These findings suggest that guanosine potentiates the growth-inhibitory effect of ara-C in B16 F10 melanoma cells by increasing the intracellular concentration of its active metabolites.

Original languageEnglish
Pages (from-to)523-529
Number of pages7
JournalCancer Investigation
Volume11
Issue number5
DOIs
StatePublished - 1993

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