GSH oxidation and protein synthesis in rabbit reticulocytes

Tova Zehavi-Willner, Nechama S. Kosower, Tim Hunt, Edward M. Kosower

Research output: Contribution to journalArticlepeer-review

Abstract

Synthesis of protein in intact rabbit reticulocytes is inhibited by the thiol oxidizing agent, diamide, which oxidizes GSH to GSSG: 1. No incorporation of 14C-leucine into soluble protein is observed during the period when GSH is absent from the cells (lag period). 2. Recovery of protein synthesis occurs following regeneration of GSH. 3. The degree to which protein synthesis recovers is correlated with the length of the lag period which depends on the amount of diamide used: five minutes absence of GSH in the rabbit reticulocyte preparation permits complete recovery of protein synthesis. Ten minutes absence of GSH results in a markedly diminished rate of protein synthesis.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume40
Issue number1
DOIs
StatePublished - 13 Jul 1970
Externally publishedYes

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