Grafting of triggering sites onto lymphocytes. Requirement of multivalency in the stimulation of dinitrophenyl‐modified thymocytes by anti‐dinitrophenyl antibody

A. Ravid, A. Novogrodsky, M. Wilchek

Research output: Contribution to journalArticlepeer-review

Abstract

N4‐Dinitrophenyl‐L‐2,4‐diaminobutyric acid hydrazide was coupled to aldehyde groups generated by periodate oxidation of sialyl residues on thymocytes. Anti‐2,4‐dinitrophenyl(Dnp)antibody was found to stimulate mature, hydrocortisone‐resistant thymocytes, while it had no mitogenic effect on the immature thymocytes. In order to study the involvement of different regions of the antibody molecule in the triggering process of stimulation, the mitogenicity of various antibody fragments was also assessed. The divalent F(ab')2 was found to be a superior mitogen compared to the intact antibody when added to Dnp‐conjugated thymocytes. The monovalent Fab' and Fab fragments have no mitogenic activity indicating that cross‐linkage may be a prerequisite for stimulation.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalEuropean Journal of Immunology
Volume8
Issue number5
DOIs
StatePublished - May 1978
Externally publishedYes

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