TY - JOUR
T1 - Grafting of triggering sites onto lymphocytes. Requirement of multivalency in the stimulation of dinitrophenyl‐modified thymocytes by anti‐dinitrophenyl antibody
AU - Ravid, A.
AU - Novogrodsky, A.
AU - Wilchek, M.
PY - 1978/5
Y1 - 1978/5
N2 - N4‐Dinitrophenyl‐L‐2,4‐diaminobutyric acid hydrazide was coupled to aldehyde groups generated by periodate oxidation of sialyl residues on thymocytes. Anti‐2,4‐dinitrophenyl(Dnp)antibody was found to stimulate mature, hydrocortisone‐resistant thymocytes, while it had no mitogenic effect on the immature thymocytes. In order to study the involvement of different regions of the antibody molecule in the triggering process of stimulation, the mitogenicity of various antibody fragments was also assessed. The divalent F(ab')2 was found to be a superior mitogen compared to the intact antibody when added to Dnp‐conjugated thymocytes. The monovalent Fab' and Fab fragments have no mitogenic activity indicating that cross‐linkage may be a prerequisite for stimulation.
AB - N4‐Dinitrophenyl‐L‐2,4‐diaminobutyric acid hydrazide was coupled to aldehyde groups generated by periodate oxidation of sialyl residues on thymocytes. Anti‐2,4‐dinitrophenyl(Dnp)antibody was found to stimulate mature, hydrocortisone‐resistant thymocytes, while it had no mitogenic effect on the immature thymocytes. In order to study the involvement of different regions of the antibody molecule in the triggering process of stimulation, the mitogenicity of various antibody fragments was also assessed. The divalent F(ab')2 was found to be a superior mitogen compared to the intact antibody when added to Dnp‐conjugated thymocytes. The monovalent Fab' and Fab fragments have no mitogenic activity indicating that cross‐linkage may be a prerequisite for stimulation.
UR - http://www.scopus.com/inward/record.url?scp=0018097943&partnerID=8YFLogxK
U2 - 10.1002/eji.1830080502
DO - 10.1002/eji.1830080502
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 308451
AN - SCOPUS:0018097943
SN - 0014-2980
VL - 8
SP - 289
EP - 294
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 5
ER -