TY - JOUR
T1 - Golgi membranes are absorbed into and reemerge from the ER during mitosis
AU - Zaal, Kristien J.M.
AU - Smith, Carolyn L.
AU - Polishchuk, Roman S.
AU - Altan, Nihal
AU - Cole, Nelson B.
AU - Ellenberg, Jan
AU - Hirschberg, Koret
AU - Presley, John F.
AU - Roberts, Theresa H.
AU - Siggia, Eric
AU - Phair, Robert D.
AU - Lippincott-Schwartz, Jennifer
N1 - Funding Information:
We thank Dr. J. Bonifacino, Dr. J. Donaldson, Dr. B. Nichols, and Dr. A. Kenworthy (NIH, Bethesda, MD) for valuable comments on this manuscript. E. S. is supported by R01 GM59018-01 from NIH. We also thank Dr. S. Tao Cheng and the staff of the NINDS EM Facility for immuno-EM analysis, and L. M. Hartnell (CBMB, NIH) for helpful contributions. We also thank those who provided reagents.
PY - 1999/12/10
Y1 - 1999/12/10
N2 - Quantitative imaging and photobleaching were used to measure ER/Golgi recycling of GFP-tagged Golgi proteins in interphase cells and to monitor the dissolution and reformation of the Golgi during mitosis. In interphase, recycling occurred every 1.5 hr, and blocking ER egress trapped cycling Golgi enzymes in the ER with loss of Golgi structure. In mitosis, when ER export stops, Golgi proteins redistributed into the ER as shown by quantitative imaging in vivo and immuno-EM. Comparison of the mobilities of Golgi proteins and lipids ruled out the persistence of a separate mitotic Golgi vesicle population and supported the idea that all Golgi components are absorbed into the ER. Moreover, reassembly of the Golgi complex after mitosis failed to occur when ER export was blocked. These results demonstrate that in mitosis the Golgi disperses and reforms through the intermediary of the ER, exploiting constitutive recycling pathways. They thus define a novel paradigm for Golgi genesis and inheritance.
AB - Quantitative imaging and photobleaching were used to measure ER/Golgi recycling of GFP-tagged Golgi proteins in interphase cells and to monitor the dissolution and reformation of the Golgi during mitosis. In interphase, recycling occurred every 1.5 hr, and blocking ER egress trapped cycling Golgi enzymes in the ER with loss of Golgi structure. In mitosis, when ER export stops, Golgi proteins redistributed into the ER as shown by quantitative imaging in vivo and immuno-EM. Comparison of the mobilities of Golgi proteins and lipids ruled out the persistence of a separate mitotic Golgi vesicle population and supported the idea that all Golgi components are absorbed into the ER. Moreover, reassembly of the Golgi complex after mitosis failed to occur when ER export was blocked. These results demonstrate that in mitosis the Golgi disperses and reforms through the intermediary of the ER, exploiting constitutive recycling pathways. They thus define a novel paradigm for Golgi genesis and inheritance.
UR - http://www.scopus.com/inward/record.url?scp=0033544712&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81548-2
DO - 10.1016/S0092-8674(00)81548-2
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AN - SCOPUS:0033544712
SN - 0092-8674
VL - 99
SP - 589
EP - 601
JO - Cell
JF - Cell
IS - 6
ER -