Glycolipids of a halotolerant, moderately halophilic bacterium. Biosynthesis of glucosylphosphatidylglycerol by cell-free particles

Nora Stern; Alisa Tietz

doi:10.1016/0005-2760(78)90155-8

Glycolipids of a halotolerant, moderately halophilic bacterium. Biosynthesis of glucosylphosphatidylglycerol by cell-free particles

Nora Stern^*
, Alisa Tietz

^*Corresponding author for this work

Department of Neurobiology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A 247 000 × g particulate fraction from a moderately halophilic halotolerant bacterium incorporated [¹⁴C]glucose added as UDP[¹⁴C]glucose and ³²P-labeled phosphatidylglycerol into glucosylphosphatidylglycerol. Exogenously added phosphatidylglycerol was available to the enzyme only when dispersed in a detergent, preferably Triton X-405, by sonication. The ¹⁴C- or ³²P-labeled glucosylphosphatidylglycerol was degraded with phospholipase C. The water soluble product formed was isolated and identified by paper chromatography as glucosylglycerolphosphate. The system required Mg²⁺ or Ca²⁺ for activity. KCl and NaCl were inhibitory even when added at low concentrations.

Original language	English
Pages (from-to)	357-366
Number of pages	10
Journal	Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume	530
Issue number	3
DOIs	https://doi.org/10.1016/0005-2760(78)90155-8
State	Published - 28 Sep 1978

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abstract = "A 247 000 × g particulate fraction from a moderately halophilic halotolerant bacterium incorporated [14C]glucose added as UDP[14C]glucose and 32P-labeled phosphatidylglycerol into glucosylphosphatidylglycerol. Exogenously added phosphatidylglycerol was available to the enzyme only when dispersed in a detergent, preferably Triton X-405, by sonication. The 14C- or 32P-labeled glucosylphosphatidylglycerol was degraded with phospholipase C. The water soluble product formed was isolated and identified by paper chromatography as glucosylglycerolphosphate. The system required Mg2+ or Ca2+ for activity. KCl and NaCl were inhibitory even when added at low concentrations.",

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N2 - A 247 000 × g particulate fraction from a moderately halophilic halotolerant bacterium incorporated [14C]glucose added as UDP[14C]glucose and 32P-labeled phosphatidylglycerol into glucosylphosphatidylglycerol. Exogenously added phosphatidylglycerol was available to the enzyme only when dispersed in a detergent, preferably Triton X-405, by sonication. The 14C- or 32P-labeled glucosylphosphatidylglycerol was degraded with phospholipase C. The water soluble product formed was isolated and identified by paper chromatography as glucosylglycerolphosphate. The system required Mg2+ or Ca2+ for activity. KCl and NaCl were inhibitory even when added at low concentrations.

AB - A 247 000 × g particulate fraction from a moderately halophilic halotolerant bacterium incorporated [14C]glucose added as UDP[14C]glucose and 32P-labeled phosphatidylglycerol into glucosylphosphatidylglycerol. Exogenously added phosphatidylglycerol was available to the enzyme only when dispersed in a detergent, preferably Triton X-405, by sonication. The 14C- or 32P-labeled glucosylphosphatidylglycerol was degraded with phospholipase C. The water soluble product formed was isolated and identified by paper chromatography as glucosylglycerolphosphate. The system required Mg2+ or Ca2+ for activity. KCl and NaCl were inhibitory even when added at low concentrations.

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Glycolipids of a halotolerant, moderately halophilic bacterium. Biosynthesis of glucosylphosphatidylglycerol by cell-free particles

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