Glycolipids of a halotolerant, moderately halophilic bacterium. Biosynthesis of glucosylphosphatidylglycerol by cell-free particles

Nora Stern, Alisa Tietz

Research output: Contribution to journalArticlepeer-review

Abstract

A 247 000 × g particulate fraction from a moderately halophilic halotolerant bacterium incorporated [14C]glucose added as UDP[14C]glucose and 32P-labeled phosphatidylglycerol into glucosylphosphatidylglycerol. Exogenously added phosphatidylglycerol was available to the enzyme only when dispersed in a detergent, preferably Triton X-405, by sonication. The 14C- or 32P-labeled glucosylphosphatidylglycerol was degraded with phospholipase C. The water soluble product formed was isolated and identified by paper chromatography as glucosylglycerolphosphate. The system required Mg2+ or Ca2+ for activity. KCl and NaCl were inhibitory even when added at low concentrations.

Original languageEnglish
Pages (from-to)357-366
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume530
Issue number3
DOIs
StatePublished - 28 Sep 1978

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