TY - JOUR
T1 - Glutathione X. Human erythrocyte membrane diffusion coefficients for diazene derivatives of the dip series via intracellular thiol oxidation
AU - Kosower, Nechama S.
AU - Kosower, Edward M.
AU - Saltoun, Gilda
AU - Levi, Lilly
N1 - Funding Information:
Support from the Chief Scientist's Office, Ministry of Health, Government
PY - 1975/1/6
Y1 - 1975/1/6
N2 - The thiol-oxidizing agent, DIP(diazenedicarboxylic acid bis (N′-methylpiperazide), I) and its homologs with different N'alkyl groups can be utilized to obtain membrane diffusion coefficients for human erythrocytes at 1°. From the initial rates of intracellular glutathione (GSH) oxidation and the 2-octanol partition coefficients for the specific DIP homolog, diffusion coefficients of ca. 1 × 10-8cm2/sec are obtained. The method (irreversible loss of permeant through well-defined intracellular reaction) can be used to evaluate membrane properties and possibly for the study of substituent effects on drug entry into cells.
AB - The thiol-oxidizing agent, DIP(diazenedicarboxylic acid bis (N′-methylpiperazide), I) and its homologs with different N'alkyl groups can be utilized to obtain membrane diffusion coefficients for human erythrocytes at 1°. From the initial rates of intracellular glutathione (GSH) oxidation and the 2-octanol partition coefficients for the specific DIP homolog, diffusion coefficients of ca. 1 × 10-8cm2/sec are obtained. The method (irreversible loss of permeant through well-defined intracellular reaction) can be used to evaluate membrane properties and possibly for the study of substituent effects on drug entry into cells.
UR - http://www.scopus.com/inward/record.url?scp=0016433807&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(75)80410-4
DO - 10.1016/S0006-291X(75)80410-4
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AN - SCOPUS:0016433807
SN - 0006-291X
VL - 62
SP - 98
EP - 102
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -