TY - JOUR
T1 - Glutathione. V. The effects of the thiol-oxidizing agent diamide on initiation and translation in rabbit reticulocytes
AU - Zehavi-Willner, Tova
AU - Kosower, Edward M.
AU - Hunt, Tim
AU - Kosower, Nechama S.
N1 - Funding Information:
The authors are grateful for support from the National Institutes of Health, the Army Research Office (Durham) and the Petroleum Research Fund (PRF-3695-AI). N.S.K. is the recipient of a Career Development Award of the National Institutes of Health.
PY - 1971/1/1
Y1 - 1971/1/1
N2 - 1. 1. Addition of the thiol-oxidizing agent, diamide ((CH3)2NCON=NCON(CH3)2), to rabbit reticulocytes, which are actively synthesizing protein, halts instantaneously the incorporation of labeled amino acid into soluble protein along with the oxidation of glutathione (GSH) to the disulfide (GSSG). 2. 2. Both translation and initiation are affected. 3. 3. Processes involved in translation (and release) recover after a short lag, following regeneration of 40-60 % of the original GSH. 4. 4. Initiation is more sensitive than translation to treatment with diamide, recovers after regeneration of 70-80 % of the original GSH, but after long periods of zero GSH, only incompletely. 5. 5. The rapidity of the response of the protein synthesizing system to diamide treatment suggests that thiol groups are intimately and directly linked to various stages in protein synthesis. 6. 6. The remarkable reversibility observed in protein synthesis after diamide treatment offers a new probe into the details of the complex process.
AB - 1. 1. Addition of the thiol-oxidizing agent, diamide ((CH3)2NCON=NCON(CH3)2), to rabbit reticulocytes, which are actively synthesizing protein, halts instantaneously the incorporation of labeled amino acid into soluble protein along with the oxidation of glutathione (GSH) to the disulfide (GSSG). 2. 2. Both translation and initiation are affected. 3. 3. Processes involved in translation (and release) recover after a short lag, following regeneration of 40-60 % of the original GSH. 4. 4. Initiation is more sensitive than translation to treatment with diamide, recovers after regeneration of 70-80 % of the original GSH, but after long periods of zero GSH, only incompletely. 5. 5. The rapidity of the response of the protein synthesizing system to diamide treatment suggests that thiol groups are intimately and directly linked to various stages in protein synthesis. 6. 6. The remarkable reversibility observed in protein synthesis after diamide treatment offers a new probe into the details of the complex process.
UR - http://www.scopus.com/inward/record.url?scp=0014986310&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(71)90564-8
DO - 10.1016/0005-2787(71)90564-8
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AN - SCOPUS:0014986310
SN - 0005-2787
VL - 228
SP - 245
EP - 251
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -